Enhanced rat sciatic nerve regeneration through silicon tubes filled with pyrroloquinoline quinone
Enhanced rat sciatic nerve regeneration through silicon tubes filled with pyrroloquinoline quinone
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DOI：
10.1002/micr.20126
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发表时间：
2005-01-01
期刊：
影响因子：
2.1
通讯作者：
Yang, JW
中科院分区：
文献类型：
--
作者：
Liu, SQ;Li, HH;Yang, JW
吡咯喹啉醌（PQQ）是一种抗氧化剂，也刺激神经生长因子（NGF）的合成和分泌。在我们实验室早期的初步研究中，雪旺细胞生长加速，NGF mRNA表达和NGF分泌促进。本研究旨在探讨PQQ对1cm节段性缺损神经管模型的可能神经诱导作用。在大鼠模型中建立8 mm坐骨神经缺损，并通过1 cm硅胶管桥接。PQQ组在硅胶腔灌注0.03mmol/L PQQ 10 μ l。对照组输注等量生理盐水。每只动物每隔2周进行功能观察（SFI），每隔4周进行电生理学研究，持续12周。在实验结束时，即管植入后12周，进行组织学和形态计量学分析。使用数字图像分析系统，测量髓鞘的厚度，并计数再生轴突的总数。PQQ组神经再生的SFI、电生理指标（运动神经传导速度和活动电位幅度）和形态计量学结果（再生轴突数和髓鞘厚度）与对照组比较差异有统计学意义（P <0.05）。PQQ组神经再生较成熟，密度较高，新生神经较多。我们的结论是，PQQ是一个有效的促进周围神经再生。(c)2005 Wiley-Liss，Inc.
Pyrroloquinoline quinone (PQQ) is an antioxidant that also stimulates nerve growth factor (NGF) synthesis and secretion. In an earlier pilot study in our laboratory, Schwann cell growth was accelerated, and NGF mRNA expression and NGF secretion were promoted. The present study was designed to explore the possible nerve-inducing effect of PQQ on a nerve tube model over a 1-cm segmental deficit. An 8-mm sciatic nerve deficit was created in a rat model and bridged by a 1-cm silicone tube. Then, 10 mu l of 0.03 mmol/I PQQ were perfused into the silicone chamber in the PQQ group. The same volume of normal saline was delivered in the control group. Each animal underwent functional observation (SFI) at 2-week intervals and electrophysiological studies at 4-week intervals for 12 weeks. Histological and morphometrical analyses were performed at the end of the experiment, 12 weeks after tube implantation. Using a digital image-analysis system, thickness of the myelin sheath was measured, and total numbers of regenerated axons were counted. There was a significant difference in SFI, electrophysiological index (motor-nerve conduct velocity and amplitude of activity potential), and morphometrical results (regenerated axon number and thickness of myelin sheath) in nerve regeneration between the PQQ group and controls (P < 0.05). More mature, high-density, newly regenerated nerve was observed in the PQQ group. We conclude that PQQ is a potent enhancer for the regeneration of peripheral nerves. (c) 2005 Wiley-Liss, Inc.