Distinguishing Leishmania tropica and Leishmania major in the Middle East using the polymerase chain reaction with kinetoplast DNA-specific primers.
Distinguishing Leishmania tropica and Leishmania major in the Middle East using the polymerase chain reaction with kinetoplast DNA-specific primers.
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DOI：
10.1016/s0035-9203(02)90057-x
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发表时间：
2002-04-01
影响因子：
2.2
通讯作者：
Greenblatt, Charles L
中科院分区：
文献类型：
--
作者：
Anders, Gerlind;Eisenberger, Carol L;Greenblatt, Charles L
本文报道了试图开发一种敏感和廉价的程序，快速诊断皮肤利什曼病的物种水平上使用皮肤刮片从病人。结果表明，该地区存在3种利什曼原虫（Leishmaniamajor，L. tropica和L.婴儿）在以色列和约旦河西岸表明，需要一个物种特异性检测方法在这一地区。引物Uni 21/Lmj 4是在L.主要的小环序列，但它也扩增其他'旧世界'物种的利什曼原虫。由于微环大小的物种特异性差异，这些引物可用于聚合酶链反应，以回答诊断和流行病学问题。
This paper reports attempts to develop a sensitive and inexpensive procedure for rapid diagnosis of cutaneous leishmaniasis at the species level using skin scrapings from patients. The presence of 3 species (Leishmania major, L. tropica and L. infantum) in Israel and the West Bank demonstrates the need for a species-specific detection method in this region. The primer pair Uni21/Lmj4 was developed on the basis of an L. major minicircle sequence but it also amplified other 'Old World' species of Leishmania. Due to species-specific differences in the size of minicircles, these primers can be used in the polymerase chain reaction to answer diagnostic and epidemiological questions.