Chromium oligopeptide activates insulin receptor tyrosine kinase activity
Chromium oligopeptide activates insulin receptor tyrosine kinase activity
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DOI：
10.1021/bi963154t
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发表时间：
1997-04-15
期刊：
影响因子：
2.9
通讯作者：
Vincent, JB
中科院分区：
文献类型：
--
作者：
Davis, CM;Vincent, JB
一个可能的新机制，放大胰岛素受体酪氨酸激酶活性，以响应胰岛素已被确定。含铬寡肽低分子量铬结合物质(LMWCR)在无胰岛素的情况下不影响大鼠脂肪细胞膜片段的酪氨酸蛋白激酶活性，但膜片段中的胰岛素刺激的蛋白激酶活性可被低分子铬结合物质提高8倍。利用分离的大鼠胰岛素受体，LMWCR被证明与胰岛素激活的胰岛素受体结合，其解离常数约为250 pm，导致其酪氨酸蛋白激酶活性增加。低分子量铬刺激胰岛素受体酪氨酸激酶活性的能力依赖于其铬含量。这些结果似乎解释了之前鲜为人知的铬与成人发病的糖尿病和心血管疾病之间的关系。
A possible new mechanism for the amplification of insulin receptor tyrosine kinase activity in response to insulin has been identified. The chromium-containing oligopeptide low molecular weight chromium-binding substance (LMWCr) does not effect the tyrosine protein kinase activity of rat adipocytic membrane fragments in the absence of insulin; however, insulin-stimulated kinase activity in the membrane fragments is increased up to 8-fold by the oligopeptide. Using isolated rat insulin receptor, LMWCr has been shown to bind to insulin-activated insulin receptor with a dissociation constant of circa 250 pM, resulting in the increase of its tyrosine protein kinase activity. The ability of LMWCr to stimulate insulin receptor tyrosine kinase activity is dependent on its chromium content. The results appear to explain the previously poorly understood relationship between chromium and adult-onset diabetes and cardiovascular disease.