Analyzing Characteristic Length Scales in Biofilm Structures
Analyzing Characteristic Length Scales in Biofilm Structures
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DOI：
10.1002/bit.22075
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发表时间：
2009-02-01
影响因子：
3.8
通讯作者：
Morgenroth, E.
中科院分区：
文献类型：
--
作者：
Milferstedt, K.;Pos, M-N.;Morgenroth, E.
基于图像分析的生物膜结构的量化需要像代表性元素区域（REA）的统计测量，以确定待成像的生物膜区域的必要尺寸。在这项研究中，生物膜结构的REAs计算的描述符灰度和相关性（COR）来自空间灰度依赖矩阵分析（SGLDM）。这两个描述符之间的一个重要区别是它们对不同空间尺度的结构特征的反应。灰度是与尺度无关的描述符，而COR是与尺度相关的。对于尺度依赖描述符，当确定REA时，各个图像的大小是不相关的。这与尺度依赖性描述符相反，对于尺度依赖性描述符，仅当每个图像的区域覆盖生物膜的结构可变性的范围时才能确定REA。我们使用COR来分析不同长度尺度下结构异质性的尺度依赖性。生物膜图像中400 μ m的特征长度提供了与生物膜中的传质现象相关的结构信息。灰度级和COR的总体REA平均为3.4 mm（2）。尺度相关的描述符COR不能在所有情况下，准确地确定从结合个别的图像分析结果，即使当合并的区域导致的REA。显微镜和相机规格定义了可从图像中提取的可检测特征长度的上限和下限，因此在选择成像技术时应在实验设计中考虑。
The quantification of biofilm structure based on image analysis requires a statistical measure like representative elemental areas (REA) to determine the necessary size of biofilm area to be imaged. In this study, REAs for biofilm structure were calculated for the descriptors Gray level and Correlation (COR) derived from a spatial gray level dependence matrix analysis (SGLDM). An important difference between these two descriptors is their response to structural features at different spatial scales. Gray level is a scale-independent descriptor, whereas COR is scale-dependent. For scale-in dependent descriptors, the size of the individual images is not relevant when determining REAs. This is in contrast to scale-dependent descriptors for which REAs can only be determined when the area of each image covers the range of structural variability of the biofilm. We used COR to analyze scale dependence of structural heterogeneity at different length scales. A characteristic length of 400 mu m in biofilm images provides structural information relevant for mass transport phenomena in biofilms. Overall REAs for gray level and COR were on average 3.4 mm(2). The scale-dependent descriptor COR could not in all cases accurately be determined from combining individual image analysis results-even when the combined area resulted in the REA. Microscope and camera specifications define the upper and lower limit of detectable characteristic length that can be extracted from images and should therefore be considered in the experimental design when choosing an imaging technique.