Assessment of the Biological Similarity of Three Capsaicin Analogs (Capsinoids) Found in Non-Pungent Chili Pepper (CH-19 Sweet) Fruits
Assessment of the Biological Similarity of Three Capsaicin Analogs (Capsinoids) Found in Non-Pungent Chili Pepper (CH-19 Sweet) Fruits
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DOI：
10.1271/bbb.90570
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发表时间：
2010-02-01
影响因子：
1.6
通讯作者：
Takahashi, Michio
中科院分区：
文献类型：
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作者：
Sasahara, Ikuko;Furuhata, Yasufumi;Takahashi, Michio
CH-19甜椒是一种新发现的辣椒品种，果实辛辣得多。由于CH-19甜水果中含有辣椒素的三种类似物(辣椒素)，辣椒素是辣椒辣味的主要成分(类似物有辣椒素或CST，二氢辣椒素或DCT，去甲二氢辣椒素或NDCT)，我们在本研究中通过与辣椒素的比较来评估这三种辣椒素的生物效价。与瞬时电位香草素1(TRPV1)受体结合的三种衣壳蛋白在培养的细胞中表达，并以浓度依赖的方式激活钙内流，其幅度相似。与辣椒素相反，相同浓度的辣椒素在小鼠的眼睛或口腔中几乎不会引起伤害性反应。给大鼠静脉注射辣椒素或增加20倍剂量的辣椒素可显著增加血浆儿茶酚胺水平。口服后，每种仙人掌都能增加小鼠的耗氧量。根据目前的结果，辣椒素和这三个辣椒素类化合物应该具有类似的生物效力，尽管辣椒素类化合物不会产生刺激性或感官刺激。
CH-19 Sweet is a newly found chili pepper breed bearing much less pungent fruits. Because CH-19 Sweet fruits were found to contain three analogs (capsinoids) of capsaicin, a major component of pungency of hot peppers (the analogs are capsiate or CST, dihydrocapsiate or DCT, and nordihydrocapsiate or NDCT), we assessed in this study the bio-potencies of these three capsinoids by comparing them with capsaicin. The three capsinoids bound to transient potential vanilloid 1 (TRPV1) receptors expressed in cultured cells and activated Ca2+ influx in a concentration-dependent manner with similar magnitudes. In contrast to capsaicin, capsinoids at the same concentration induced virtually no nociceptive responses when applied to the eyes or the oral cavities of mice. Intravenous administration of capsaicin or 20-fold increased doses of each capsinoid to rats induced significant increases in plasma catecholamine levels. Orally administered, each capsinoid enhanced oxygen consumption in mice. Based on the present results, capsaicin and these three capsinoids should have similar bio-potency, though capsinoids do not generate pungency or sensory irritation.