INITIATION OF SPORULATION IN BACILLUS-SUBTILIS IS CONTROLLED BY A MULTICOMPONENT PHOSPHORELAY
INITIATION OF SPORULATION IN BACILLUS-SUBTILIS IS CONTROLLED BY A MULTICOMPONENT PHOSPHORELAY
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DOI：
10.1016/0092-8674(91)90238-t
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发表时间：
1991-02-08
期刊：
影响因子：
64.5
通讯作者：
HOCH, JA
中科院分区：
文献类型：
--
作者：
BURBULYS, D;TRACH, KA;HOCH, JA
枯草芽孢杆菌孢子形成0期（spo 0）突变体在启动孢子形成的信号转导系统中存在缺陷。这些基因的两种产物Spo0A和Spo0F与用于控制细菌环境反应的双组分调节系统的反应调节剂组分有关。Spo0F反应调节剂被发现是孢子形成特异性蛋白激酶KinA磷酸化的主要底物。磷酸化Spo0F是磷酸转移酶Spo0B的磷酸供体，该磷酸转移酶将磷酸基团转移到第二个反应调节因子，转录调节蛋白Spo0A。这种磷酸化中继提供了一种机制，使用Spo0F作为第二信使从几种蛋白激酶收集信号。这些不同的信号通过Spo0B磷酸转移酶整合以激活Spo0A转录因子。该系统提供了许多水平的控制，以防止孢子形成的反复无常的诱导。
Stage 0 sporulation (spo0) mutants of Bacillus subtilis are defective in the signal transduction system initiating sporulation. Two of the products of these genes, Spo0A and Spo0F, are related to response regulator components of two-component regulatory systems used to control environmental responses in bacteria. The Spo0F response regulator was found to be the primary substrate for phosphorylation by the sporulation-specific protein kinase, KinA. Phosphorylated Spo0F was the phosphodonor for a phosphotransferase, Spo0B, which transferred the phosphate group to the second response regulator, the transcription regulatory protein Spo0A. This phosphorelay provides a mechanism for signal gathering from several protein kinases using Spo0F as a secondary messenger. These divergent signals are integrated through Spo0B phosphotransferase to activate the Spo0A transcription factor. This system provides for many levels of control to prevent capricious induction of sporulation.