Bone Morphogenetic Protein 4 Inhibits Cell Proliferation and Induces Apoptosis in Glioma Stem Cells
Bone Morphogenetic Protein 4 Inhibits Cell Proliferation and Induces Apoptosis in Glioma Stem Cells
复制标题
骨形态发生蛋白4抑制神经胶质瘤干细胞增殖并诱导细胞凋亡
DOI：
10.1089/cbr.2010.0857
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发表时间：
2011-02-01
影响因子：
3.4
通讯作者：
Liu, Ning
中科院分区：
文献类型：
--
作者：
Zhou, Zhimin;Sun, Lihua;Liu, Ning
胶质瘤干细胞（Glioma stem cells，GSC）是神经胶质瘤的肿瘤起始细胞，是中枢神经系统最常见的原发性恶性肿瘤。大量研究表明，骨形态发生蛋白4（BMP 4）在神经干细胞的分化和增殖中起着重要作用。为探讨BMP 4在GSCs中的作用及其机制，采用长春新碱诱导分离的U87胶质瘤细胞，将其与BMP 4蛋白共同作用。本研究表明，BMP 4通过下调cyclin D1水平抑制U87 GSC增殖（p < 0.01），并通过诱导Bax表达和抑制Bcl-2和Bcl-xL水平促进GSC凋亡。因此，这些结果提示了一种基于GSC的胶质瘤治疗的新方法。
Glioma stem cells (GSCs), which are originated from transformed neural stem cells, are tumor-initiating cells of glioma, the most common primary malignant neoplasm of the central nervous system. Extensive studies have shown that bone morphogenetic protein 4 (BMP4) plays an important role in the differentiation and proliferation of neural stem cells. To seek the functions and mechanisms of BMP4 in GSCs, GSCs isolated from U87 human glioma cells by using vincristine were exposed to BMP4 protein. This study shows that BMP4 inhibited U87 GSC proliferation (p < 0.01) via downregulation of cyclin D1 level and promoted GSC apoptosis through induction of Bax expression and inhibition of Bcl-2 and Bcl-xL levels. Thus, these results indicate a new approach of GSC-based glioma treatment.