Generation of dendritic cells from lentiviral vector-transduced CD34+ cells from HIV+ donors.
Generation of dendritic cells from lentiviral vector-transduced CD34+ cells from HIV+ donors.
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从 HIV 供体的慢病毒载体转导的 CD34 细胞生成树突状细胞。
DOI：
10.1002/jmv.10376
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
Wong-Staal,F
中科院分区：
文献类型：
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作者：
Gruber,A;Chen,I;Kuhen,KL;Wheat,JC;Law,P;Wong-Staal,F
树突状细胞有望成为癌症和感染性疾病免疫治疗的佐剂。我们证明，大量来自HIV感染受试者的冻存外周血CD 34+细胞可以用表达HIV抗原的复制缺陷型慢病毒载体转导。此外，来自HIV感染者的未转导和转导的CD 34+细胞能够分化为具有强T细胞刺激能力的树突状细胞。因此，来自HIV感染受试者的冻存CD 34+细胞可能被证明可用于HIV/AIDS的免疫治疗。医学病毒学杂志70：183-186，2003.© 2003 Wiley利斯公司
Dendritic cells hold promise as adjuvant for immunotherapy for cancer and infectious diseases. We demonstrate that a significant number of cryopreserved peripheral blood CD34+cells from HIV‐infected subjects can be transduced with a replication‐incompetent lentiviral vector expressing HIV antigens. In addition, untransduced and transduced CD34+cells from HIV‐infected individuals were able to differentiate into dendritic cells with strong T‐cell stimulatory capacity. Thus, cryopreserved CD34+cells from HIV‐infected subjects may prove useful for immunotherapy for HIV/AIDS. J. Med. Virol. 70: 183–186, 2003. © 2003 Wiley‐Liss, Inc.