DesT Coordinates the Expression of Anaerobic and Aerobic Pathways for Unsaturated Fatty Acid Biosynthesis in Pseudomonas aeruginosa
DesT Coordinates the Expression of Anaerobic and Aerobic Pathways for Unsaturated Fatty Acid Biosynthesis in Pseudomonas aeruginosa
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DOI：
10.1128/jb.00404-09
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发表时间：
2010-01-01
影响因子：
3.2
通讯作者：
Zhang, Yong-Mei
中科院分区：
文献类型：
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作者：
Subramanian, Chitra;Rock, Charles O.;Zhang, Yong-Mei
Faba和Fabb基因负责铜绿假单胞菌厌氧不饱和脂肪酸的形成。外源不饱和脂肪酸抑制FabAB操纵子的表达，并利用翻译起始点上游的DNA序列亲和纯化DeST。在DEST缺失菌株中，野生型细胞提取物中检测到的单个蛋白与FabAB启动子之间没有相互作用，不饱和脂肪酸对FabAB表达的抑制也是如此。因此，DEST感知酰基辅酶A池的总体组成，以协调不饱和脂肪酸合成的厌氧(FabAB)和有氧(DesCB)途径的操纵子的表达。
The fabA and fabB genes are responsible for anaerobic unsaturated fatty acid formation in Pseudomonas aeruginosa. Expression of the fabAB operon was repressed by exogenous unsaturated fatty acids, and DNA sequences upstream of the translational start site were used to affinity purify DesT. The single protein interaction with the fabAB promoter detected in wild-type cell extracts was absent in the desT deletion strain, as was the repression of fabAB expression by unsaturated fatty acids. Thus, DesT senses the overall composition of the acyl-coenzyme A pool to coordinate the expression of the operons for the anaerobic (fabAB) and aerobic (desCB) pathways for unsaturated fatty acid synthesis.