A stress-specific calcium signature regulating an ozone-responsive gene expression network in Arabidopsis.
A stress-specific calcium signature regulating an ozone-responsive gene expression network in Arabidopsis.
复制标题
DOI：
10.1111/j.1365-313x.2012.05043.x
复制
发表时间：
2012-09
期刊：
影响因子：
--
通讯作者：
E. Short;K. A. North;M. Roberts;A. Hetherington;A. Shirras;M. McAinsh
中科院分区：
文献类型：
--
作者：
E. Short;K. A. North;M. Roberts;A. Hetherington;A. Shirras;M. McAinsh
基因表达的变化是植物适应和响应环境胁迫的分子机制的关键组成部分。有令人信服的证据表明刺激特异性Ca（2+）信号在植物胁迫反应中的作用。然而，我们对它们如何协调应激诱导基因的差异表达的理解仍然是碎片化的。为了测试Ca（2+）信号编码刺激特异性转录信息的能力，我们对污染臭氧诱导的拟南芥转录组变化进行了一项全球研究。我们发现一组臭氧诱导基因的表达是依赖于Ca（2+）的，并且臭氧诱导的Ca（2+）信号的消除抑制了臭氧对这些基因的诱导。在臭氧产生的活性氧（ROS）之一H(2)O(2)或同样产生ROS的冷胁迫(两者都刺激[Ca（2+)]（cyt）的变化）时，未观察到这组臭氧调节基因的诱导。这些数据明确地表明，在臭氧作用下观察到的Ca（2+）依赖性基因表达变化不仅仅是ros诱导的[Ca(2+)]（cyt）本身增加的结果。臭氧、H(2)O(2)和冷Ca（2+）特征的大小和时间动态都有显著差异。这一发现与植物中复杂Ca（2+）信号的时空动态可以编码刺激特异性转录信息的假设一致。
Changes in gene expression form a key component of the molecular mechanisms by which plants adapt and respond to environmental stresses. There is compelling evidence for the role of stimulus-specific Ca(2+) signatures in plant stress responses. However, our understanding of how they orchestrate the differential expression of stress-induced genes remains fragmentary. We have undertaken a global study of changes in the Arabidopsis transcriptome induced by the pollutant ozone in order to establish a robust transcriptional response against which to test the ability of Ca(2+) signatures to encode stimulus-specific transcriptional information. We show that the expression of a set of co-regulated ozone-induced genes is Ca(2+)-dependent and that abolition of the ozone-induced Ca(2+) signature inhibits the induction of these genes by ozone. No induction of this set of ozone-regulated genes was observed in response to H(2)O(2), one of the reactive oxygen species (ROS) generated by ozone, or cold stress, which also generates ROS, both of which stimulate changes in [Ca(2+)](cyt). These data establish unequivocally that the Ca(2+)-dependent changes in gene expression observed in response to ozone are not simply a consequence of an ROS-induced increase in [Ca(2+) ](cyt) per se. The magnitude and temporal dynamics of the ozone, H(2)O(2) , and cold Ca(2+) signatures all differ markedly. This finding is consistent with the hypothesis that stimulus-specific transcriptional information can be encoded in the spatiotemporal dynamics of complex Ca(2+) signals in plants.