The DELLA proteins interact with MYB21 and MYB24 to regulate filament elongation in Arabidopsis
The DELLA proteins interact with MYB21 and MYB24 to regulate filament elongation in Arabidopsis
复制标题
DELLA 蛋白与 MYB21 和 MYB24 相互作用来调节拟南芥中的丝伸长
DOI：
10.1186/s12870-020-2274-0
复制
发表时间：
2020-02-07
影响因子：
5.3
通讯作者：
Song, Susheng
中科院分区：
文献类型：
--
作者：
Huang, Huang;Gong, Yilong;Song, Susheng
背景
赤霉素(GA)和茉莉酸(JA)是拟南芥花丝伸长所必需的两种植物激素。GA和JA分别通过SCFSLY1和SCFCOI1激活DELLAS和JASMOATE ZIM结构域(JAZ)蛋白的降解，激活纤维伸长。在JA途径中，JAZ与MYB21和MYB24相互作用，控制细丝的伸长。然而，人们对德拉斯如何调节细丝伸长知之甚少。
结果
这里我们显示了Dellas与MYB21和MYB24相互作用，MYB21和MYB24的R2R3结构域负责与Dellas相互作用。此外，我们还证明了Della和Jaz蛋白协同抑制MYB21和MYB24的转录功能，从而抑制了细丝的伸长。
结论
我们发现Dellas与MYB21和MYB24相互作用，并且Dellas和JAZ减弱了MYB21和MYB24的转录功能，以控制细丝伸长。本研究揭示了GA和JA在调节拟南芥花丝伸长中的一种新的串扰机制。
Background
Gibberellin (GA) and jasmonate (JA) are two essential phytohormones for filament elongation in Arabidopsis. GA and JA trigger degradation of DELLAs and JASMONATE ZIM-domain (JAZ) proteins through SCFSLY1 and SCFCOI1 separately to activate filament elongation. In JA pathway, JAZs interact with MYB21 and MYB24 to control filament elongation. However, little is known how DELLAs regulate filament elongation.
Results
Here we showed that DELLAs interact with MYB21 and MYB24, and that R2R3 domains of MYB21 and MYB24 are responsible for interaction with DELLAs. Furthermore, we demonstrated that DELLA and JAZ proteins coordinately repress the transcriptional function of MYB21 and MYB24 to inhibit filament elongation.
Conclusion
We discovered that DELLAs interact with MYB21 and MYB24, and that DELLAs and JAZs attenuate the transcriptional function of MYB21 and MYB24 to control filament elongation. This study reveals a novel cross-talk mechanism of GA and JA in the regulation of filament elongation in Arabidopsis.