Treatment with β2-adrenoceptor agonist in vivo induces human clock gene, Per1, mRNA expression in peripheral blood
Treatment with β2-adrenoceptor agonist in vivo induces human clock gene, Per1, mRNA expression in peripheral blood
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DOI：
10.1080/07420520601140043
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发表时间：
2007-01-01
影响因子：
2.8
通讯作者：
Shimizu, Eiji
中科院分区：
文献类型：
--
作者：
Burioka, Naoto;Takata, Miyako;Shimizu, Eiji
本研究检测了体内暴露于82肾上腺素能受体激动剂妥洛特罗是否会诱导外周血细胞中人周期[liPerl] mRNA的表达。在一项实验中，5名健康志愿者在22:00 h口服妥洛特罗，在另一项实验中，同样的5名受试者在20:00 h口服妥洛特罗透皮贴片。在每个实验中，在四个时间点测量血清妥洛特罗浓度，并从外周血细胞中分离总RNA，通过实时聚合酶链反应检测hPer1 mRNA的表达。妥洛布特罗片和透皮贴片均增加了hPerl mRNA的表达，表明对人外周血细胞的分析可以可靠地代表体内外周时钟基因mRNA的表达。
This study examined whether in vivo exposure to a 82-adrenoceptor agonist, tulobuterol, induces human Period] (liPerl) mRNA expression in cells from peripheral whole blood. In one experiment, oral tulobuterol was administered to five healthy volunteers at 22:00 h, while in another, a transdermally tulobuterol patch was applied to the same five subjects at 20:00 h. In each experiment, serum tulobuterol concentrations were measured at four time points, and total RNA was isolated from periphcral blood cells for determinations of hPer1 mRNA expression by real-time polymerase chain reaction. Both the tulobuterol tablet and the transdermal patch increased hPerl mRNA expression, suggesting that analyses of human peripheral blood cells could reliably represent peripheral clock gene mRNA expression in vivo.