Hormonal and developmental regulation of adrenodoxin messenger ribonucleic acid in steroidogenic tissues.
Hormonal and developmental regulation of adrenodoxin messenger ribonucleic acid in steroidogenic tissues.
复制标题
类固醇生成组织中肾上腺素氧还蛋白信使核糖核酸的激素和发育调节。
DOI：
--
复制
发表时间：
1988
影响因子：
5.8
通讯作者：
W. Miller
中科院分区：
文献类型：
--
作者：
R. Voutilainen;J. Picado;A. Diblasio;W. Miller
肾上腺氧还蛋白是一种在类固醇生成组织的线粒体中发现的铁硫蛋白。它作为线粒体细胞色素 P450（包括胆固醇侧链裂解酶 P450scc）的电子传递中间体参与类固醇生成。使用我们实验室最近克隆的人类肾上腺氧还蛋白 cDNA 探针，我们检查了肾上腺氧还蛋白 mRNA 在各种类固醇生成组织中的分布和激素调节。在所有检查的类固醇生成组织中都发现了肾上腺氧还蛋白 mRNA。在人类胎儿睾丸中，肾上腺氧还蛋白 mRNA 在妊娠早期更为丰富，到中期逐渐减少，其模式与我们之前报道的 P450scc 非常相似。然而，与 P450scc 不同的是，在人类胎儿卵巢中检测到大量肾上腺素氧还蛋白 mRNA，没有明显的妊娠依赖性变化。通过 hCG、FSH、cAMP 和霍乱毒素处理，培养的人颗粒细胞中肾上腺氧还蛋白 mRNA 的丰度增加。在人胎儿肾上腺细胞中，ACTH和cAMP刺激肾上腺素还蛋白mRNA的积累，而在培养的人胎儿睾丸细胞和培养的胎儿恒河猴卵巢细胞中，hCG和cAMP均刺激肾上腺素还素mRNA的积累。在所有这些系统中，肾上腺氧还蛋白 mRNA 的积累与 P450scc 的反应密切相关。这些数据表明这些功能相关但结构不相关的蛋白质的基因以协调的方式受到调节。
Adrenodoxin is an iron-sulfur protein found in the mitochondria of steroidogenic tissues. It participates in steroidogenesis as an electron transport intermediate for mitochondrial cytochromes P450, including P450scc, the cholesterol side-chain cleavage enzyme. Using a human adrenodoxin cDNA probe recently cloned in our laboratory, we examined the distribution and hormonal regulation of adrenodoxin mRNA in a variety of steroidogenic tissues. Adrenodoxin mRNA was found in all steroidogenic tissues examined. In human fetal testes, adrenodoxin mRNA was more abundant in early gestation, diminishing toward midterm in a pattern closely similar to that we reported previously for P450scc. Unlike P450scc, however, significant amounts of adrenodoxin mRNA were detected in human fetal ovaries, with no discernible gestation-dependent change. The abundance of adrenodoxin mRNA was increased in cultured human granulosa cells by treatment with hCG, FSH, cAMP, and cholera toxin. In human fetal adrenal cells, ACTH and cAMP stimulated accumulation of adrenodoxin mRNA, while in cultured human fetal testicular cells and cultured fetal rhesus monkey ovarian cells, both hCG and cAMP stimulated accumulation of adrenodoxin mRNA. In all of these systems, the accumulation of adrenodoxin mRNA closely paralleled the response of P450scc. These data suggest that the genes for these functionally related but structurally unrelated proteins are regulated in a coordinate manner.
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Yanagibashi,K;Haniu,M;Shively,JE;Shen,WH;Hall,P
通讯作者：
Hall,P
牛肾上腺素还蛋白前体形式的分子克隆和氨基酸序列：先前未识别的 COOH 末端肽的证据。
DOI：
10.1073/pnas.82.17.5705
发表时间：
1985
影响因子：
11.1
作者：
Okamura,T;John,ME;Zuber,MX;Simpson,ER;Waterman,MR
通讯作者：
Waterman,MR