Adipocyte accumulation in penile corpus cavernosum of the orchiectomized rabbit: a potential mechanism for veno-occlusive dysfunction in androgen deficiency.
Adipocyte accumulation in penile corpus cavernosum of the orchiectomized rabbit: a potential mechanism for veno-occlusive dysfunction in androgen deficiency.
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DOI：
10.1002/j.1939-4640.2005.tb01091.x
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发表时间：
2005-03
影响因子：
--
通讯作者：
A. Traish;P. Toselli;Seong-Joo Jeong;Noel N Kim
中科院分区：
文献类型：
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作者：
A. Traish;P. Toselli;Seong-Joo Jeong;Noel N Kim
雄激素被认为对阴茎组织的发育、生长和维持以及勃起功能至关重要。据报道，雄激素还能抑制基质祖细胞分化为脂肪细胞，并促进其分化为平滑肌。本研究的目的是探讨雄激素剥夺是否会导致海绵体中脂肪细胞的积聚。将成年新西兰白兔雄性分为假手术组（对照组）和去势组。手术后两周，通过监测海绵体内血压（ICP）对盆腔神经刺激的反应来评估勃起功能。所有ICP测量值均以平均全身动脉血压进行标准化。在平行研究中，将对照组和去势组兔子的阴茎横切面固定，并用Masson三色染色法或苏木精 - 伊红染色法来评估平滑肌和结缔组织含量。或者，用甲苯胺蓝对组织切片染色以评估含脂肪细胞的积聚情况。去势导致勃起功能丧失和阴茎萎缩，同时伴有小梁平滑肌减少和结缔组织含量增加。最显著的是，去势动物的组织在海绵体的内膜下区域出现了含脂肪细胞（脂肪细胞）的积聚。我们假设雄激素剥夺促进基质祖细胞分化为产生含脂肪细胞的脂肪生成谱系，从而改变勃起功能。
Androgens are deemed to be critical for the development, growth, and maintenance of penile tissue as well as for erectile function. Androgens are also reported to inhibit differentiation of stroma progenitor cells into adipocytes and promote differentiation into smooth muscle. The objective of this study was to investigate whether androgen deprivation results in accumulation of adipocytes in the corpus cavernosum. Mature, New Zealand white male rabbits were subjected to sham surgery (control) or orchiectomy. Two weeks after surgery, erectile function was assessed by monitoring changes in intracavernosal blood pressure (ICP) in response to pelvic nerve stimulation. All ICP measurements were normalized to the mean systemic arterial blood pressure. In parallel studies, penile cross sections from control and orchiectomized rabbits were fixed and stained with either Masson's trichrome or hematoxylin and eosin to assess smooth muscle and connective tissue content. Alternatively, tissue sections were stained with Toluidine blue to assess accumulation of fat-containing cells. Orchiectomy resulted in loss of erectile function and penile atrophy, associated with reduced trabecular smooth muscle and increased connective tissue content. Most strikingly, tissue from orchiectomized animals exhibited accumulation of fat-containing cells (adipocytes) in the subtunical region of the corpus cavernosum. We hypothesize that androgen deprivation promotes differentiation of progenitor stroma cells into an adipogenic lineage producing fat-containing cells, thus altering erectile function.