Whole-body protein anabolic response is resistant to the action of insulin in obese women
Whole-body protein anabolic response is resistant to the action of insulin in obese women
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DOI：
10.1093/ajcn/82.2.355
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发表时间：
2005-08-01
影响因子：
7.1
通讯作者：
Gougeon, R
中科院分区：
文献类型：
--
作者：
Chevalier, S;Marliss, EB;Gougeon, R
背景：肥胖与糖脂代谢胰岛素抵抗有关。目的：我们试图确定肥胖对蛋白质代谢胰岛素敏感性的影响。设计：在吸收后状态和高胰岛素、高血糖、异氨基酸钳夹期间，对9名瘦女性和10名肥胖女性的全身[C-13]亮氨酸和[3h]葡萄糖动力学进行了测量。结果：在吸收后状态下，肥胖者的亮氨酸内源性出现率（分解代谢率）比瘦子高11%，非氧化消失率（合成）比瘦子高8%，净平衡比瘦子高29% （P < 0.05）。肥胖女性的钳形氨基酸和葡萄糖输注率显著低于瘦女性(分别为0.65 +/- 0.02和0.85 +/- 0.04,5.7 +/- 0.3和9.1 +/- 0.5 mg (.) kg无脂质量（-1）和.)min(-1))；P < 0.0001)，二者的发病率呈正相关（r = 0.635, P = 0.005）。在高胰岛素血症期间，肥胖女性的合成刺激较少，净亮氨酸平衡远低于瘦女性（分别为-0.08 +/- 0.06和0.30 +/- 0.03 mu mol. kg无脂质量（-1）（.）min(-1)）；P < 0.0001)。净亮氨酸平衡百分比变化与各肥胖指数呈负相关。血浆游离脂肪酸受到的抑制程度较低，肥胖妇女的呼吸商低于苗条妇女。结论：肥胖妇女对高胰岛素血症表现出迟钝的蛋白质合成代谢反应，这与胰岛素对蛋白质作用的抵抗以及对糖脂代谢的抵抗是一致的。
Background: Obesity is associated with insulin resistance of glucose and lipid metabolism.Objective: We sought to determine the effects of obesity on the insulin sensitivity of protein metabolism. Design: Whole-body [C-13]leucine and [3 H]glucose kinetics were measured in 9 lean and 10 obese women in the postabsorptive state and during a hyperinsulinemic, euglycemic, isoaminoacidemic clamp.Results: In the postabsorptive state, the leucine endogenous rate of appearance (catabolism), normalized for fat-free mass, was 11% greater and the nonoxidative rate of disappearance (synthesis) was 8% greater in the obese than in the lean women, but net balance was 29% more negative (P < 0.05). Clamp amino acid and glucose infusion rates were significantly lower in the obese women than in the lean women (0.65 +/- 0.02 compared with 0.85 +/- 0.04 and 5.7 +/- 0.3 compared with 9.1 +/- 0.5 mg (.) kg fat-free mass(-1) (.) min(-1), respectively; P < 0.0001 for both), and their rates correlated positively (r = 0.635, P = 0.005). During hyperinsulinemia, synthesis was stimulated less and net leucine balance was much lower in the obese women than in the lean women (-0.08 +/- 0.06 and 0.30 +/- 0.03 mu mol. kg fat-free mass(-1) (.) min(-1), respectively; P < 0.0001). The percentage change in net leucine balance correlated negatively with all adiposity indexes. Plasma free fatty acids were less suppressed and the respiratory quotient was lower in the obese women than in the lean women.Conclusion: Obese women show a blunted protein anabolic response to hyperinsulinemia that is consistent with resistance to the action of insulin on protein concurrent with that on glucose and lipid metabolism.