Study on membrane recycling in the rat visceral yolk-sac endoderm using concanavalin-A conjugates
Study on membrane recycling in the rat visceral yolk-sac endoderm using concanavalin-A conjugates
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使用刀豆球蛋白-A 缀合物研究大鼠内脏卵黄囊内胚层的膜回收
DOI：
10.1007/bf00684383
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
A. Miki
中科院分区：
文献类型：
--
作者：
P. Kugler;A. Miki
本文用辣根过氧化物酶和铁蛋白标记的伴刀豆球蛋白A（Con-A HRP，Con-A Fer）研究了大鼠内脏卵黄囊培养（全胚胎培养;孕10.5天培养24小时）的内胚层细胞中顶细胞膜物质的内化和胞内运动。当内脏卵黄囊内胚层在4°C下暴露于Con-A HRP或Con-A Fer 5 min时，含有发育良好的绒毛被的顶端细胞膜被大量标记，而顶端空泡、溶酶体和顶端小管则没有。Con-A标记的内胚层在20° C和37°C无Con-A血清中孵育5 - 60 min，导致膜结合凝集素进入包被囊泡、顶端空泡和溶酶体的温度依赖性内化，并且顶端细胞膜清除了重标记。随着培养时间的延长，标记的液泡结构的数量和标记强度逐渐减少，而标记的顶端小管的数量增加。因此，在37°C下30和60分钟后，大多数顶小管含有高浓度的标记物。可以观察到标记的顶端小管，其与标记的顶端空泡和溶酶体以及顶端细胞膜连续。大鼠内胚层细胞中的这些发现表明，顶端细胞膜的成分在顶端空泡和溶酶体中被内化，然后通过顶端小管被带回顶端细胞膜，顶端小管浓缩和储存这种膜材料。
SummaryThe internalization and intracellular movements of apical-cell-membrane material were investigated in the endodermal cells of cultured visceral yolk-sacs of rats (whole-embryo culture; explanted at 10.5 days of gestation and cultured for 24h) using horseradish peroxidase- and ferritin-labelled concanavalin A (Con-A HRP, Con-A Fer). When visceral yolk-sac endoderm was exposed to Con-A HRP or Con-A Fer for 5 min at 4°C, the apical cell membranes containing a well-developed fuzzy coat were heavily labelled, whereas apical vacuoles, lysosomes and apical canaliculi were not. Incubation of Con-A-labelled endoderm for 5 60 min at 20° and 37°C in Con-A-free serum resulted in a temperature-dependent internalization of membranebound lectin into coated vesicles, apical vacuoles and lysosomes, and the apical cell membranes were cleared of the heavy labelling. With increasing incubation time, the number of labelled vacuolar structures and the intensity of their labelling decreased gradually, whereas the number of labelled apical canaliculi increased. Thus, after 30 and 60 min at 37°C, most of the apical canaliculi contained high concentrations of the markers. It was possible to observe labelled apical canaliculi that were in continuity with labelled apical vacuoles and lysosomes as well as with the apical cell membrane. These findings in rat endodermal cells indicate that constitutents of the apical cell membrane are internalized in apical vacuoles and lysosomes, and are then brought back to the apical cell membrane by the apical canaliculi, which concentrate and store this membrane material.
包被囊泡在跨卵黄囊上皮选择性转移中的作用。
DOI：
10.1016/s0022-5320(82)90003-x
发表时间：
1982
期刊：
Journal of ultrastructure research
影响因子：
--
作者：
King,BF
通讯作者：
King,BF