Adherence to Mediterranean diet is associated with methylation changes in inflammation-related genes in peripheral blood cells
Adherence to Mediterranean diet is associated with methylation changes in inflammation-related genes in peripheral blood cells
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DOI：
10.1007/s13105-017-0552-6
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发表时间：
2016-08-01
影响因子：
3.4
通讯作者：
Martinez, J. A.
中科院分区：
文献类型：
--
作者：
Arpon, A.;Riezu-Boj, J. I.;Martinez, J. A.
表观遗传过程，包括 DNA 甲基化，可能会受到饮食等环境因素的调节，而饮食又与肥胖或心血管事件等多种疾病的发生有关。与此同时，地中海饮食（MedDiet）已被证明对心血管风险、血压、炎症和其他与过度肥胖相关的并发症有良好的效果。其中一些效应可能是通过表观遗传修饰介导的。因此，本研究的目的是调查坚持 MedDiet 是否与外周血细胞甲基化状态的变化相关。 Prevencion con Dieta Mediterranea (PREDIMED)-Navarra 研究中选择了 36 名受试者，这是一项随机、对照、平行试验，对高心血管风险志愿者进行三组干预，其中两组采用地中海饮食，一组低脂对照组。研究了基线和 5 年之间甲基化的变化。 DNA 甲基化阵列通过多项可靠的统计测试和功能分类进行分析。最终选择了与炎症和免疫功能相关的 8 个基因（EEF2、COL18A1、IL4I1、LEPR、PLAGL1、IFRD1、MAPKAPK2、PPARGC1B），因为它们的甲基化水平变化与 MedDiet 的依从性相关，并且它们表现出与甲基化变化的高变异性相关的敏感性。此外，EEF2 甲基化水平与 TNF-α 和 CRP 浓度呈正相关。这份报告显然是第一个表明坚持 MedDiet 与所报道的与炎症相关的基因的甲基化有关，并具有潜在的调节影响。
Epigenetic processes, including DNA methylation, might be modulated by environmental factors such as the diet, which in turn have been associated with the onset of several diseases such as obesity or cardiovascular events. Meanwhile, Mediterranean diet (MedDiet) has demonstrated favourable effects on cardiovascular risk, blood pressure, inflammation and other complications related to excessive adiposity. Some of these effects could be mediated by epigenetic modifications. Therefore, the objective of this study was to investigate whether the adherence to MedDiet is associated with changes in the methylation status from peripheral blood cells. A subset of 36 individuals was selected within the Prevencion con Dieta Mediterranea (PREDIMED)-Navarra study, a randomised, controlled, parallel trial with three groups of intervention in high cardiovascular risk volunteers, two with a MedDiet and one low-fat control group. Changes in methylation between baseline and 5 years were studied. DNA methylation arrays were analysed by several robust statistical tests and functional classifications. Eight genes related to inflammation and immunocompetence (EEF2, COL18A1, IL4I1, LEPR, PLAGL1, IFRD1, MAPKAPK2, PPARGC1B) were finally selected as changes in their methylation levels correlated with adherence to MedDiet and because they presented sensitivity related to a high variability in methylation changes. Additionally, EEF2 methylation levels positively correlated with concentrations of TNF-alpha and CRP. This report is apparently the first showing that adherence to MedDiet is associated with the methylation of the reported genes related to inflammation with a potential regulatory impact.