High-resolution gene expression atlases for adult and developing mouse brain and spinal cord
High-resolution gene expression atlases for adult and developing mouse brain and spinal cord
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DOI：
10.1007/s00335-012-9406-2
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发表时间：
2012-10-01
期刊：
影响因子：
2.5
通讯作者：
Hohmann, John G.
中科院分区：
文献类型：
--
作者：
Henry, Alex M.;Hohmann, John G.
随着世界各地的研究实验室对大脑功能各个方面的探索，对哺乳动物大脑在正常和病理状态下的结构、遗传学、电路和生理特性的了解不断增加。然而，直到最近，中枢神经系统的基因表达的全面编目一直缺乏。作为推动神经科学研究的使命的一部分，艾伦脑科学研究所已经完成了几个大型的小鼠、非人类灵长类动物和人类大脑基因图谱项目，提供了信息丰富的在线公共资源和工具。在这里，我们提出了艾伦小鼠大脑图谱，涵盖整个成年小鼠大脑的20，000个基因;艾伦发育小鼠大脑图谱，详细介绍了大脑生长的7个胚胎和出生后阶段的大约2，000个重要发育基因的表达;和艾伦脊髓图谱，揭示了成年和新生小鼠脊髓中20，000个基因的表达。综合数据挖掘工具，包括参考地图集，信息分析，和3-D观众，进行了说明。对于这些大规模的项目，开发了高通量工业技术，以标准化和可靠地重复实验目标。为了验证一致性和准确性，通过将我们的数据与同行评议的文献和其他数据库进行比较，对成年小鼠大脑的1,000个最受欢迎的基因进行了详细分析（根据网站页面浏览量）。我们表明，我们的数据与独立来源高度一致，并提供了每月数千名研究人员使用的信息和工具的综合汇编。所有数据和工具都可以通过艾伦大脑图谱门户网站（www.brain-map.org）免费获得。
Knowledge of the structure, genetics, circuits, and physiological properties of the mammalian brain in both normal and pathological states is ever increasing as research labs worldwide probe the various aspects of brain function. Until recently, however, comprehensive cataloging of gene expression across the central nervous system has been lacking. The Allen Institute for Brain Science, as part of its mission to propel neuroscience research, has completed several large gene-mapping projects in mouse, nonhuman primate, and human brain, producing informative online public resources and tools. Here we present the Allen Mouse Brain Atlas, covering 20,000 genes throughout the adult mouse brain; the Allen Developing Mouse Brain Atlas, detailing expression of approximately 2,000 important developmental genes across seven embryonic and postnatal stages of brain growth; and the Allen Spinal Cord Atlas, revealing expression for 20,000 genes in the adult and neonatal mouse spinal cords. Integrated data-mining tools, including reference atlases, informatics analyses, and 3-D viewers, are described. For these massive-scale projects, high-throughput industrial techniques were developed to standardize and reliably repeat experimental goals. To verify consistency and accuracy, a detailed analysis of the 1,000 most viewed genes for the adult mouse brain (according to website page views) was performed by comparing our data with peer-reviewed literature and other databases. We show that our data are highly consistent with independent sources and provide a comprehensive compendium of information and tools used by thousands of researchers each month. All data and tools are freely available via the Allen Brain Atlas portal (www.brain-map.org).