In vitro differentiation of epithelial cells cultured from human periodontal ligament
In vitro differentiation of epithelial cells cultured from human periodontal ligament
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DOI：
10.1111/j.1600-0765.2007.00969.x
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发表时间：
2007-10-01
影响因子：
3.5
通讯作者：
Komatsu, M.
中科院分区：
文献类型：
--
作者：
Shimonishi, M.;Hatakeyama, J.;Komatsu, M.
背景和目的：碱性磷酸酶和非胶原骨蛋白在牙骨质形成之前产生。虽然已有研究表明Malassez上皮细胞与牙骨质形成有关，但对Malassez上皮细胞与牙骨质形成的关系知之甚少。本研究的目的是探讨体外培养的人牙周膜细胞上皮细胞是否能产生碱性磷酸酶和骨桥蛋白、骨钙素、骨涎蛋白等非胶原性骨蛋白。材料和方法：从牙周膜外植体中扩增出一种可能的细胞上皮细胞，并进行第二代培养。以人牙龈上皮细胞和牙周膜成纤维细胞为对照。免疫组织化学染色和原位杂交分析釉原蛋白的表达水平。结果：在Malassez细胞上皮细胞中存在高水平的釉原蛋白、碱性磷酸酶和骨桥蛋白及其相应的mRNAs。骨钙素和骨涎蛋白在可能的马氏细胞上皮细胞中不表达。结论：单独培养的Malassez细胞不能转化为成熟的细胞形成牙骨质，但在牙周膜成纤维细胞中可能起到一定的矿化作用。
Background and Objective: Alkaline phosphatase and noncollagenous bone proteins are produced prior to cementum formation. While it has been suggested that epithelial rests of Malassez are involved in cementum formation, little is known about the relationship between epithelial rests of Malassez and cementum formation. The purpose of the present study was to determine whether the epithelial rests of Malassez cells cultured from human periodontal ligament can produce alkaline phosphatase and noncollagenous bone proteins, such as osteopontin, osteocalcin and bone sialoprotein.Material and Methods: An outgrowth of putative epithelial rests of Malassez cells was produced from periodontal ligament explant, and second passage cultures were used in the experiments. Human gingival epithelial cells and periodontal ligament fibroblasts were used as controls. The expression levels of amelogenin were analyzed by immunostaining and in situ hybridization. Furthermore, the expression levels of alkaline phosphatase and noncollagenous bone proteins were assessed by immunostaining and reverse transcription-polymerase chain reaction.Results: Amelogenin, alkaline phosphatase and osteopontin proteins and their corresponding mRNAs were detected at high levels in putative epithelial rests of Malassez cells. Osteocalcin and bone sialoprotein were not expressed in putative epithelial rests of Malassez cells. Alkaline phosphatase and noncollagenous bone proteins were seen in periodontal ligament fibroblasts, but not in gingival epithelial cells.Conclusion: Our results suggest that putative epithelial rests of Malassez cells cultured alone do not transform into maturing cells to form the cementum, but may play a potential role in the mineralization process.