Stiffness and Oligomer Content Affect the Initial Adhesion of Staphylococcus aureus to Polydimethylsiloxane Gels
Stiffness and Oligomer Content Affect the Initial Adhesion of Staphylococcus aureus to Polydimethylsiloxane Gels
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DOI：
10.1021/acsami.3c11349
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发表时间：
2023-11-03
影响因子：
9.5
通讯作者：
Schiffman，Jessica D.
中科院分区：
文献类型：
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作者：
Barajas，Brandon;Kurtz，Irene S.;Schiffman，Jessica D.
耐甲氧西林金黄色葡萄球菌（S。金黄色葡萄球菌）感染需要更好地理解它们对医学相关表面的初始粘附。研究了聚二甲基硅氧烷（PDMS）凝胶的力学性能和低聚物含量对革兰氏阳性菌初始附着的影响。探索了金。进行小振幅振荡剪切流变学测量以验证通过改变常用Sylgard 184硅酮弹性体试剂盒（B：C比率为60：1、40：1、10：1和5：1）的基础与固化（B：C）比率，可以合成具有跨越四种不同状态的杨氏模量的PDMS凝胶：超软（15 kPa）、软（30 kPa）、标准（400 kPa）和硬（1500 kPa）。将这些制备的凝胶（未提取的）与由相同B/C比率制备的凝胶进行索氏提取以除去任何未反应的低聚物进行比较。虽然从相同B：C比例制备的未浸提和浸提PDMS凝胶的杨氏模量在统计学上相等，但浸提后超软凝胶的相关粘附失效能量在统计学上降低（从25 J/mm 2降至8 J/mm 2）。使用S测试了这八种表征良好的凝胶与细菌的相互作用。aureusSH 1000，一种常用的实验室研究菌株，以及S. aureusATCC 12600，其分离自人肺部感染。增加S。只有当细菌直接在以高B：C比例（40：1和60：1）制备的未提取凝胶上温育时，才发生金黄色葡萄球菌灭活，而提取的凝胶（没有未反应的低聚物）都没有显著水平的灭活细菌。金黄色葡萄球菌粘附到最硬的提取的PDMS凝胶上最少（没有未反应的低聚物），而粘附到软的未提取的PDMS凝胶上最多（具有约17%的未反应的低聚物）。这些发现表明，未反应的低聚物和杨氏模量是探索革兰氏阳性菌的附着行为时要考虑的重要材料因素。金黄色疏水性弹性体凝胶。
The growing prevalence of methicillin-resistantStaphylococcus aureus(S. aureus) infections necessitates a greater understanding of their initial adhesion to medically relevant surfaces. In this study, the influence of the mechanical properties and oligomer content of polydimethylsiloxane (PDMS) gels on the initial attachment of Gram-positiveS. aureuswas explored. Small-amplitude oscillatory shear rheological measurements were conducted to verify that by altering the base to curing (B:C) ratio of the commonly used Sylgard 184 silicone elastomer kit (B:C ratios of 60:1, 40:1, 10:1, and 5:1), PDMS gels could be synthesized with Young’s moduli across four distinct regimes: ultrasoft (15 kPa), soft (30 kPa), standard (400 kPa), and stiff (1500 kPa). These as-prepared gels (unextracted) were compared to gels prepared from the same B/C ratios that underwent Soxhlet extraction to remove any unreacted oligomers. While the Young’s moduli of unextracted and extracted PDMS gels prepared from the same B:C ratio were statistically equivalent, the associated adhesion failure energy statistically decreased for the ultrasoft gels after extraction (from 25 to 8 J/mm2). The interactions of these eight well-characterized gels with bacteria were tested by usingS. aureusSH1000, a commonly studied laboratory strain, as well asS. aureusATCC 12600, which was isolated from a human lung infection. IncreasedS. aureusinactivation occurred only when the bacteria were incubated directly on top of the unextracted gels prepared at high B:C ratios (40:1 and 60:1), whereas none of the extracted gels (no unreacted oligomers) had significant levels of inactivated bacteria.S. aureusadhered the least to the stiffest extracted PDMS gels (no unreacted oligomers) and the most to soft, unextracted PDMS gels (with ∼17% unreacted oligomers). These findings suggest that both unreacted oligomers and Young’s moduli are important material factors to consider when exploring the attachment behavior of Gram-positiveS. aureusto hydrophobic elastomer gels.