Amplified DNA in Y1 mouse adrenal tumor cells: isolation of cDNAs complementary to an amplified c-Ki-ras gene and localization of homologous sequences to mouse chromosome 6.
Amplified DNA in Y1 mouse adrenal tumor cells: isolation of cDNAs complementary to an amplified c-Ki-ras gene and localization of homologous sequences to mouse chromosome 6.
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Y1 小鼠肾上腺肿瘤细胞中的 DNA 扩增：分离与扩增的 c-Ki-ras 基因互补的 cDNA，并将同源序列定位到小鼠 6 号染色体。
DOI：
10.1093/nar/12.6.2731
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发表时间：
1984
影响因子：
14.9
通讯作者：
Francke,U
中科院分区：
文献类型：
--
作者：
George,DL;Scott,AF;deMartinville,B;Francke,U
我们已经分离出与在Y1小鼠肾上腺肿瘤细胞中扩增的c-Ki-ras细胞癌基因互补的cDNA克隆，扩增的序列位于双微体染色质体（DM）和均匀染色的染色体区域（HSR）。cDNA的表征包括相应基因组克隆的分离、RNA的北方印迹分析和DNA序列分析。我们的研究表明，在Y1细胞中扩增的c-Ki-ras基因与人c-Ki-ras 2基因同源。我们还获得的证据表明，除了c-Ki-ras，至少有一个其他的转录单位已在小鼠肾上腺肿瘤细胞扩增。此外，通过Southern杂交分析，我们确定了与DM和HSR相关的扩增DNA序列，包括c-Ki-ras基因，存在于正常小鼠6号染色体上
We have isolated cDNA clones complementary to a c-Ki-rascellular oncogene that is amplified in Yl mouse adrenal tumor cells, with the amplified sequences located on double-minute chromatin bodies (DMs) and homogeneously staining chromosomal regions (HSRs). Characterization of the cDNAs included the isolation of corresponding genomic clones, Northern blot analysis of RNA, and DNA sequence analysis. Our studies demonstrate that the c-Ki-rasgene amplified in the Yl cells is homologous to the human c-Ki-ras2gene. We have also obtained evidence that, in addition to c-Ki-ras, at least one other transcription unit has been amplified in the mouse adrenal tumor cells. Moreover, by Southern blot analysis of Chinese hamster-mouse somatic cell hybrids, we have determined that the amplified DNA sequences associated with DMs and HSRs, including the c-Ki-rasgene, are present in normal mouse cells on chromosome 6