Microfluidic flow cytometry: The role of microfabrication methodologies, performance and functional specification.
Microfluidic flow cytometry: The role of microfabrication methodologies, performance and functional specification.
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DOI：
10.1142/s2339547818300019
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Yarmush ML
中科院分区：
文献类型：
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作者：
Shrirao AB;Fritz Z;Novik EM;Yarmush GM;Schloss RS;Zahn JD;Yarmush ML
流式细胞术是在现代生物医学研究和临床应用中使用的非常宝贵的工具，其需要高通量、高分辨率的颗粒分析以用于细胞计数表征和/或细胞和颗粒的分选以及用于分析来自免疫细胞计数测定的结果。近年来，研究集中在开发微流体流式细胞仪，其动机是创建更小、更便宜、更简单和更自主的传统流式细胞仪的替代品。这些设备理想地可以是高度便携的，易于操作而无需广泛的用户培训，并且用于研究目的和/或护理点诊断，特别是在资源有限的设施或需要现场分析的位置中。然而，设计一种满足高通量分析、自动化和便携性的标准，同时不牺牲性能的设备不是一件小事。本文旨在介绍该领域的现状，并通过重点介绍微流控流式细胞仪的关键设计组件，为进一步改进提供考虑。粒子聚焦和检测策略的最新创新进行了详细的比较。本文综述了流式细胞仪的性能矩阵参数是相互依赖的，建议权衡选择的基础上的应用程序的要求。微流控技术的持续贡献表明，它是一种可行的技术，可以推进流式细胞术的现状，并开发自动化，易于操作和具有成本效益的流式细胞仪。
Flow cytometry is an invaluable tool utilized in modern biomedical research and clinical applications requiring high throughput, high resolution particle analysis for cytometric characterization and/or sorting of cells and particles as well as for analyzing results from immunocytometric assays. In recent years, research has focused on developing microfluidic flow cytometers with the motivation of creating smaller, less expensive, simpler, and more autonomous alternatives to conventional flow cytometers. These devices could ideally be highly portable, easy to operate without extensive user training, and utilized for research purposes and/or point-of-care diagnostics especially in limited resource facilities or locations requiring on-site analyses. However, designing a device that fulfills the criteria of high throughput analysis, automation and portability, while not sacrificing performance is not a trivial matter. This review intends to present the current state of the field and provide considerations for further improvement by focusing on the key design components of microfluidic flow cytometers. The recent innovations in particle focusing and detection strategies are detailed and compared. This review outlines performance matrix parameters of flow cytometers that are interdependent with each other, suggesting trade offs in selection based on the requirements of the applications. The ongoing contribution of microfluidics demonstrates that it is a viable technology to advance the current state of flow cytometry and develop automated, easy to operate and cost-effective flow cytometers.