Continuous Glucose Monitoring in Female NOD Mice Reveals Daily Rhythms and a Negative Correlation With Body Temperature
Continuous Glucose Monitoring in Female NOD Mice Reveals Daily Rhythms and a Negative Correlation With Body Temperature
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DOI：
10.1210/en.2017-00266
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发表时间：
2017-09-01
期刊：
影响因子：
4.8
通讯作者：
Rizzo, Stacey J. Sukoff
中科院分区：
文献类型：
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作者：
Korstanje, Ron;Ryan, Jennifer L.;Rizzo, Stacey J. Sukoff
先前在小鼠中进行的连续葡萄糖监测研究仅限于几天或几周，小鼠对设备的物理附着会影响行为和测量。在目前的研究中，我们使用无线遥测技术在一组NOD/ShiLtJ雌性小鼠中以10秒的间隔测量血糖和体温，持续12周。这使我们能够获得这两个参数的昼夜节律的高分辨率曲线，以及真实的高血糖发展的开始。最引人注目的观察结果是夜间葡萄糖浓度升高，在昼夜葡萄糖升高（历史上测量葡萄糖浓度时）前几天进入糖尿病范围，以及血糖浓度升高与体温之间的强负相关性，随着糖尿病的发展，体温稳步下降。总而言之，这一技术进步为小鼠模型中糖尿病疾病轨迹的研究提供了更高的分辨率，包括与目前经常用于患者的持续葡萄糖监测技术的相关可转换性。
Previous studies with continuous glucose monitoring in mice have been limited to several days or weeks, with the mouse's physical attachment to the equipment affecting behavior and measurements. In the current study, we measured blood glucose and body temperature at 10-second intervals for 12 weeks in a cohort of NOD/ShiLtJ female mice using wireless telemetry. This allowed us to obtain a high-resolution profile of the circadian rhythm of these two parameters and the onset of hyperglycemic development in real time. The most striking observations were the elevated nocturnal concentrations of glucose into the diabetic range days before elevations in diurnal glucose (when glucose concentrations are historically measured) and the strong, negative correlation between elevated blood glucose concentrations and body temperature with a steady decline of the body temperature with diabetes development. Taken together, this technological advancement provides improved resolution in the study of the disease trajectory of diabetes in mouse models, including relevant translatability to the current technologies of continuous glucose monitoring now regularly used in patients.