Determinants of differential liver-colonizing potential of variants of the MCA-38 murine colon cancer cell line.
Determinants of differential liver-colonizing potential of variants of the MCA-38 murine colon cancer cell line.
复制标题
MCA-38 鼠结肠癌细胞系变体的不同肝脏定植潜力的决定因素。
DOI：
10.1007/bf00133619
复制
发表时间：
1995
影响因子：
4
通讯作者：
Lance,P
中科院分区：
文献类型：
--
作者：
Piscatelli,JJ;Cohen,SA;Berenson,CS;Lance,P
We investigated factors that might contribute to the differing liver tumor colonizing potentials of MCA-38 colonic cancer cell line variants injected into the ileocolic veins of C57B1/6J mice. Non-colonizing (MCA-38 CD) cells were sensitive to lysis by hepatic ntural killer (NK) cellsin vitro(51Cr-release assay) and cells with high liver-colonizing potential (MCA-38 LD) were resistant. Following abrogation of NK activity by treatment with anti-asialoGM1, liver-colonizing ability of LD cells but not CD cells was enhanced. MCA-38 CD cells were, however, capable of initial liver colonization after ileocolic vein injection. Differing patterns of membrane sialylation may have contributed to the contrasting hepatic tumorigenicities of LD and CD cells; β-galactoside α2,6-sialyltransferase mRNA levels and activity were ∼ four-fold higher in LD than CD cells and qualitative and quantitative differences existed between their ganglioside profiles. In the MCA-38 model outlined, tumor cell susceptibility or resistance to NK lysis was a relatively unimportant determinant of liver-colonizing potential.
