A hierarchical transcriptional network controls appressorium-mediated plant infection by the rice blast fungus Magnaporthe oryzae
A hierarchical transcriptional network controls appressorium-mediated plant infection by the rice blast fungus Magnaporthe oryzae
复制标题
DOI：
10.1101/2020.02.05.936203
复制
发表时间：
2020-02
期刊：
影响因子：
--
通讯作者：
Míriam Osés-Ruiz;Magdalena Martin-Urdiroz;D. Soanes;M. J. Kershaw;Neftaly Cruz-Mireles;G. Valdovinos-Ponce;Camilla Molinari;George R. Littlejohn;P. Derbyshire;Frank L. H. Menke;B. Valent;N. Talbot
中科院分区：
文献类型：
--
作者：
Míriam Osés-Ruiz;Magdalena Martin-Urdiroz;D. Soanes;M. J. Kershaw;Neftaly Cruz-Mireles;G. Valdovinos-Ponce;Camilla Molinari;George R. Littlejohn;P. Derbyshire;Frank L. H. Menke;B. Valent;N. Talbot
稻瘟病是一种普遍存在的毁灭性疾病，威胁着世界各地的水稻生产。尽管它对全球粮食安全的重要性，但是，对稻瘟病菌的植物感染的潜在生物学仍然知之甚少。特别是，目前还不清楚真菌如何精心制作一个专门的感染细胞，附着胞，以响应来自水稻叶片的表面信号。在这里，我们报告的时间协同调节的转录因子，Pmk1丝裂原活化蛋白激酶途径的下游，以调节基因表达在附着胞介导的植物感染的网络的识别。我们已经在功能上表征了这个网络的转录因子，并展示了一个分层的转录控制系统的操作。我们发现，这种分层的调节机制涉及Pmk1依赖磷酸化的Hox7同源框转录因子，抑制菌丝相关的基因表达，同时诱导附着胞发育所需的主要生理变化，包括细胞周期阻滞，自噬细胞死亡，肿胀生成和黑色素生物合成。Mst12然后调节参与隔蛋白依赖的细胞骨架重组，极化胞吐作用和植物组织入侵所必需的效应基因表达的基因功能。
Rice blast is a pervasive and devastating disease that threatens rice production across the world. In spite of its importance to global food security, however, the underlying biology of plant infection by the blast fungus Magnaporthe oryzae remains poorly understood. In particular, it is unclear how the fungus elaborates a specialised infection cell, the appressorium, in response to surface signals from the rice leaf. Here, we report the identification of a network of temporally co-regulated transcription factors that act downstream of the Pmk1 mitogen-activated protein kinase pathway to regulate gene expression during appressorium-mediated plant infection. We have functionally characterised this network of transcription factors and demonstrated the operation of a hierarchical transcriptional control system. We show that this tiered regulatory mechanism involves Pmk1-dependent phosphorylation of the Hox7 homeobox transcription factor, which represses hyphal-associated gene expression and simultaneously induces major physiological changes required for appressorium development, including cell cycle arrest, autophagic cell death, turgor generation and melanin biosynthesis. Mst12 then regulates gene functions involved in septin-dependent cytoskeletal re-organisation, polarised exocytosis and effector gene expression necessary for plant tissue invasion.