Spot compliant neuronal networks by structure optimized micro-contact printing
Spot compliant neuronal networks by structure optimized micro-contact printing
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DOI：
10.1016/s0142-9612(00)00379-3
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发表时间：
2001-07-01
期刊：
影响因子：
14
通讯作者：
Offenhäusser, A
中科院分区：
文献类型：
--
作者：
Lauer, L;Klein, C;Offenhäusser, A
体外神经细胞生长可以用细胞外基质蛋白如层粘连蛋白的微图案化结构来控制。这项技术是研究神经元细胞功能的有力工具，以提高实验的可重复性，并专门设计创新的实验装置。在本文中，ECM图案的结构尺寸和由此产生的蜂窝网络的形状之间的相关性进行了分析。本研究的目的是尽可能精确地定位神经元细胞体并诱导定向细胞分化。PCC 7-MzN细胞在层粘连蛋白模式下培养。细胞粘附位点之间的线宽、节点尺寸和间隙尺寸系统地变化。拍摄样品的显微照片，并使用Student t检验和线性相关方法进行统计学分析。精确的细胞定位已经成功地进行，并控制神经元极化的证据已经found. With结构的几何形状为4 μ m的线宽，20 μ m的节点大小和10 μ m的间隙大小的节点符合性的86%（+/- 10%）已经实现。(C)2001爱思唯尔科技有限公司版权所有。
Neuronal cell growth in vitro can be controlled with micropatterned structures of extracellular matrix proteins such as laminin. This technique is a powerful tool for studying neuronal cell function in order to increase experimental reproducibility and to specifically design innovative experimental setups. Tn this paper the correlation between the structural dimensions of the ECM pattern and the shape of the resulting cellular network is analyzed. The aim of the present study was to position neuronal cell bodies as precisely as possible and to induce directed cell differentiation. PCC7-MzN cells were cultured on laminin patterns. The line width, node size and gap size in-between cell adhesion sites was varied systematically. Micrographs of the samples were taken and statistically analyzed using Student's t-test and linear correlation methods. Precise cell positioning has successfully been performed and evidence For controlled neuronal polarization has been found. With a structure geometry of 4 mum line width, 20 mum node size and 10 mum gap size a nodal compliance of 86% (+/- 10%) has been achieved. (C) 2001 Elsevier Science Ltd. All rights reserved.