CopA:: An Escherichia coli Cu(I)-translocating P-type ATPase
CopA:: An Escherichia coli Cu(I)-translocating P-type ATPase
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DOI：
10.1073/pnas.97.2.652
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发表时间：
2000-01-18
影响因子：
11.1
通讯作者：
Rosen, BP
中科院分区：
文献类型：
--
作者：
Rensing, C;Fan, B;Rosen, BP
copA基因产物是一种假定的铜易位p型atp酶，已被证明与大肠杆菌的铜抗性有关。copA基因通过同源重组插入卡那霉素基因而被破坏。突变菌株对铜盐更敏感，但对其他金属的盐不敏感，这表明它在铜稳态中起作用。铜敏感表型可以通过携带大肠杆菌copA或霍乱肠球菌copB的质粒进行互补而恢复。铜、银盐诱导copA表达，锌、钴盐不诱导。来自表达copA的细胞的外翻膜泡表现出atp偶联的铜积累，可能是Cu(I)。结果表明，CopA是一种Cu(I)-易位外排泵，类似于与Menkes病和Wilson病相关的铜泵，为这些人类疾病提供了有用的原核模型。
The copA gene product, a putative copper-translocating P-type ATPase, has been shown to be involved in copper resistance in Escherichia coli. The copA gene was disrupted by insertion of a kanamycin gene through homologous recombination. The mutant strain was more sensitive to copper salts but not to salts of other metals, suggesting a role in copper homeostasis. The copper-sensitive phenotype could he rescued by complementation by a plasmid carrying copA from E. coli or copB from Enterococcus hirae. Expression of copA was induced by salts of copper or silver but not zinc or cobalt. Everted membrane vesicles from cells expressing copA exhibited ATP-coupled accumulation of copper, presumably as Cu(I). The results indicate that CopA is a Cu(I)-translocating efflux pump that is similar to the copper pumps related to Menkes and Wilson diseases and provides a useful prokaryotic model for these human diseases.