Prognostic significance of S100A16 subcellular localization in lung adenocarcinoma
Prognostic significance of S100A16 subcellular localization in lung adenocarcinoma
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DOI：
10.1016/j.humpath.2018.01.001
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发表时间：
2018-04-01
期刊：
影响因子：
3.3
通讯作者：
Sato, Yuichi
中科院分区：
文献类型：
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作者：
Kobayashi, Makoto;Nagashio, Ryo;Sato, Yuichi
为了发现新的肺腺癌（AC）的肿瘤标志物，我们进行了蛋白质组学分析，并报告了S100 A16在AC组织中的膜表达与不良预后之间的相关性。然而，一些预后良好的患者也显示S100 A16膜染色。我们重新评估了免疫化学染色的组织，发现在以下情况下，膜阳性和核阴性表达显著更高：男性，吸烟者，淋巴结转移阳性，p-TNM分期较高，肿瘤较大，分化较差，淋巴管浸润阳性，血管浸润阳性，胸膜浸润阳性（所有因素P <0.05）。这种染色模式也是一个独立的预后因素。此外，我们使用TCGA和Kaplan-Meier RT-PCR数据库分析了S100 A16 mRNA表达，发现AC中S100 A16 mRNA的高表达与较差的生存率显著相关。据我们所知，目前还没有针对AC患者S100 A16蛋白和mRNA表达水平的综合研究。我们的研究结果表明，S100 A16和S100 A16 mRNA表达水平的亚细胞定位是AC的一个有前途的预后标志物。(C)2018爱思唯尔公司All rights reserved.
To discover novel tumor markers for lung adenocarcinoma (AC), we performed proteomics analysis and reported a correlation between S100A16 membranous expression in AC tissues and a poor prognosis. However, some patients with a good prognosis also showed S100A16 membranous staining. We re-evaluated immunohistochemically stained tissues, and found membrane-positive and nucleus-negative expressions to be significantly higher in the presence of the following: male, smoker, positive nodal metastasis, higher p-TNM stage, larger tumor, poorer differentiation, positive for lymphatic invasion, positive for vascular invasion, and positive for pleural invasion (all factors P < .05). This pattern of staining was also an independent prognostic factor. Furthermore, we analyzed S100A16 mRNA expression using TCGA and Kaplan-Meier plotter databases, and found that higher S100A16 mRNA expression in AC was significantly correlated with poorer survival. To our knowledge, there has been no comprehensive study focused on both S100A16 protein and mRNA expression levels in AC patients. Our results suggest that the subcellular localization of S100A16 and S100A16 mRNA expression levels is a promising prognostic marker for AC. (C) 2018 Elsevier Inc. All rights reserved.