Plasma volume, cell volume, total blood volume and F factor in the tree shrew
Plasma volume, cell volume, total blood volume and F factor in the tree shrew
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树鼩的血浆量、细胞量、总血量和F因子
DOI：
10.1371/journal.pone.0234835
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发表时间：
2020-09-03
期刊：
影响因子：
3.7
通讯作者：
Tang, An-zhou
中科院分区：
文献类型：
--
作者：
Xia, Wei;Huang, Zong-jian;Tang, An-zhou
在这项研究中，血浆，细胞，总血液和F血液因子的体积的生理值确定在24个成年树（树鼩; 12男，12女;平均BW为123.9 +/- 19.19 g）。采用伊文思蓝染料二室模型法测定血浆容量，微量红细胞压积法测定静脉血红细胞压积。为了建立树鼩体重和血容量之间的关系，我们对这两个数据集进行了线性拟合。根据性别和体重（120 g）分析结果。使用非配对学生t检验和单因素ANOVA评估统计学显著性。每100 g体重的血浆、红细胞（RBC）和全血的平均体积分别为5.42 +/- 0.543、3.24 +/- 0.445和8.66 +/-0.680 ml。平均身体红细胞压积、心脏红细胞压积、颈静脉红细胞压积、股静脉红细胞压积和尾静脉红细胞压积分别为37.43 +/- 4.096、39.72 +/- 3.219、43.04 +/- 4.717、40.84 +/- 3.041和38.71 +/- 3.442%。F心脏为0.94 +/- 0.072，F颈静脉为0.88 +/- 0.118，F股静脉为0.92 +/- 0.111，F尾静脉为0.97 +/- 0.117。血容量（ml）为85.89103xBW（kg）。本研究首次提供了树鼩血浆容量、细胞容量、总血量和F因子等参数，为进一步研究树鼩血液生理提供了基础数据。
In this study, the physiological values of volumes of plasma, cells, total blood and the F blood factors were identified in 24 adult tree shrews (Tupaia belangeri; 12 male and 12 female; average BW of 123.9 +/- 19.19 g). The two-compartment model method of Evans Blue dye was used to obtain the plasma volume and the venous hematocrit was measured by microhematocrit method. To establish the relationship between body weight (BW) and blood volume of tree shrews, We performed linear fitting for these two datasets. Results were analyzed according to gender and weight (120g). Statistical significance was assessed using the unpaired student t test and one-way ANOVA. The average volumes per 100g body weight of plasma, red blood cell (RBC) and total blood were 5.42 +/- 0.543, 3.24 +/- 0.445, and 8.66 +/- 0.680ml respectively. The mean body hematocrit, cardiac hematocrit, jugular vein hematocrit, femoral vein hematocrit, and tail vein hematocrit was 37.43 +/- 4.096, 39.72 +/- 3.219, 43.04 +/- 4.717, 40.84 +/- 3.041, and 38.71 +/- 3.442% respectively. The F cardiac was 0.94 +/- 0.072, F jugular vein 0.88 +/- 0.118, F femoral vein 0.92 +/- 0.111, and the F tail vein 0.97 +/- 0.117. Blood volume (ml) was 85.89103xBW (kg). This is the first study to provide the parameters of plasma volume, cell volume, total blood volume and F factor and a baseline for future research on blood physiology of tree shrews.