Chitosan-DNA nanoparticles: The effect of cell type and hydrolysis of chitosan on in vitro DNA transfection
Chitosan-DNA nanoparticles: The effect of cell type and hydrolysis of chitosan on in vitro DNA transfection
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DOI：
10.1080/10837450600940873
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发表时间：
2006-01-01
影响因子：
3.4
通讯作者：
Nagata, Kyosuke
中科院分区：
文献类型：
--
作者：
Turan, Kadir;Nagata, Kyosuke
以稀盐酸为溶剂，在不同温度下对低分子量和中分子量壳聚糖（Ch）进行加热水解。Chs的粘均分子量随着温度的变化从450 kDa逐渐降低到14 kDa。Ch级分用于形成Ch-DNA纳米颗粒，并测试体外将DNA引入HEK 293、Swiss 3 T3、HeLa和MDCK细胞的能力。纳米粒子的平均粒径为200-220 nm。纳米颗粒的表面电荷根据Ch/DNA比率而变化。细胞株对Ch组分的DNA切割反应不同，这取决于Ch组分的分子量。HEK 293细胞被用具有宽范围分子量（类似于14-195 kDa）的Chs制备的纳米颗粒有效转染。Swiss 3 T3细胞被Ch聚合物有效转染，约
Commercial chitosan (Ch) with low (LMWCh) and medium molecular weight (MMWCh) were hydrolyzed in diluted hydrochloric acid by heating at different temperatures. The viscosity average molecular weight of Chs was gradually decreased from 450 to 14 kDa as a function of temperature. Ch fractions were used for formation of Ch-DNA nanoparticles and tested for the ability to introduce DNA into HEK293, Swiss3T3, HeLa, and MDCK cells in vitro. The average diameter of nanoparticles was 200-220 nm. The surface charge of nanoparticles varied depending on the Ch/DNA ratio. The cell lines different response to DNA transection with Ch fractions depended on molecular weight. HEK293 cells were efficiently transfected by nanoparticles prepared with Chs having a wide range of molecular weight (similar to 14-195 kDa). Swiss3T3 cells were efficiently transfected by Ch polymers with about