Aphidicolin resistant mutant of which DNA polymerase α is induced by this drug
Aphidicolin resistant mutant of which DNA polymerase α is induced by this drug
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Aphidicolin 抗性突变体，其 DNA 聚合酶 α 是由该药物诱导的
DOI：
10.1016/0006-291x(79)91970-3
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发表时间：
1979
影响因子：
3.1
通讯作者：
M. Yamada
中科院分区：
文献类型：
--
作者：
M. Nishimura;H. Yasuda;S. Ikegami;M. Ohashi;M. Yamada
从培养的小鼠乳腺癌细胞系FM 3A中筛选出对DNA聚合酶α（DNA polymerase α，DNA polymerase α）特异性抑制剂阿非迪霉素（aphidicolin）具有抗性的突变株。其中之一，命名为Aph 212，在1 μg/ml药物的存在下生长，这不允许野生型细胞生长。Aph-212细胞对阿非迪霉素的耐药性可能是由于在药物存在下培养的Aph-212细胞中DNA聚合酶α的活性增加所致。
Mutants resistant to aphidicolin, a specific inhibitor of DNA polymerase α of eukaryotic cells, were selected from cultured FM3A cells, derived from mouse mammary carcinoma. One of them, designated as Aph 212, grew in the presence of 1 μg/ml of the drug, which did not permit wild type cells to grow. The resistance of Aph 212 cells to aphidicolin seems to be due to the increment of the activity of DNA polymerase α when Aph 212 cells were cultivated in the presence of the drug.