An expanded collection of mouse Y chromosome RDA clones.
An expanded collection of mouse Y chromosome RDA clones.
复制标题
小鼠 Y 染色体 RDA 克隆的扩展集合。
DOI：
10.1007/s003359900486
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Simpson,EM
中科院分区：
文献类型：
--
作者：
Bergstrom,DE;Yan,H;Sonti,MM;Narayanswami,S;Bayleran,JK;Simpson,EM
If we are to understand the structure and function of the mammalian genome and its transmission to subsequent generations, we cannot neglect the Y Chromosome (Chr). However, certain properties of the Y have made it refractory to analysis. The bulk of the Y Chr does not recombine, and thus genetic maps based on standard Mendelian recombination are not feasible. For this reason, investigators have relied on physical approaches to map the human Y (Foote et al. 1992; Vollrath et al. 1992). We are using physical approaches to further our understanding of the mouse Y Chr by developing chromosome-specific DNA-based probes.One method for generating Y Chr-specific probes relies on subtractive molecular methodologies, which can use female (XX) genomic DNA to “subtract” away sequences from the male (XY) genome, leaving Y Chr DNA behind for further characterization. Such subtractive hybridization techniques have generally fared poorly in the context of the mammalian genome owing to its vast size and complexity, which limit the power of subtractive hybridization and subsequent enrichment.
