Detection of a Low-Grade Enteroviral Infection in the Islets of Langerhans of Living Patients Newly Diagnosed With Type 1 Diabetes
Detection of a Low-Grade Enteroviral Infection in the Islets of Langerhans of Living Patients Newly Diagnosed With Type 1 Diabetes
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DOI：
10.2337/db14-1370
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发表时间：
2015-05-01
期刊：
影响因子：
7.7
通讯作者：
Dahl-Jorgensen, Knut
中科院分区：
文献类型：
--
作者：
Krogvold, Lars;Edwin, Bjorn;Dahl-Jorgensen, Knut
糖尿病病毒检测研究（DiViD）是第一个在诊断1型糖尿病时检查新鲜胰腺组织是否存在病毒的研究。6例成年患者（年龄24-35岁）在1型糖尿病发病39周后行最小胰尾切除术。免疫组化检测肠病毒衣壳蛋白1 （VP1）的表达和ⅰ类HLA的表达。采用PCR和测序方法分析分离胰岛和新鲜冷冻全胰腺组织的肠病毒RNA。非糖尿病患者的器官捐献者作为对照。所有1型糖尿病患者（9例对照中2例）的胰岛均检测到VP1。在所有患者的胰岛中均发现I类HLA分子的高表达（9名对照组中有1名）。6例患者中有4例检测到肠道病毒特异性RNA序列（6例对照中无一例）。结果在不同的实验室得到了证实。只有1.7%的胰岛含有VP1（+）细胞，且肠道病毒RNA含量较低。结果为1型糖尿病患者胰岛中存在肠病毒提供了证据，这与胰岛中低级别肠病毒感染导致人类疾病进展的可能性是一致的。
The Diabetes Virus Detection study (DiViD) is the first to examine fresh pancreatic tissue at the diagnosis of type 1 diabetes for the presence of viruses. Minimal pancreatic tail resection was performed 39 weeks after onset of type 1 diabetes in six adult patients (age 24-35 years). The presence of enteroviral capsid protein 1 (VP1) and the expression of class I HLA were investigated by immunohistochemistry. Enterovirus RNA was analyzed from isolated pancreatic islets and from fresh-frozen whole pancreatic tissue using PCR and sequencing. Nondiabetic organ donors served as controls. VP1 was detected in the islets of all type 1 diabetic patients (two of nine controls). Hyperexpression of class I HLA molecules was found in the islets of all patients (one of nine controls). Enterovirus-specific RNA sequences were detected in four of six patients (zero of six controls). The results were confirmed in various laboratories. Only 1.7% of the islets contained VP1(+) cells, and the amount of enterovirus RNA was low. The results provide evidence for the presence of enterovirus in pancreatic islets of type 1 diabetic patients, which is consistent with the possibility that a low-grade enteroviral infection in the pancreatic islets contributes to disease progression in humans.