Krüppel-like factor 2a (KLF2A) suppresses GCRV replication by upregulating serpinc1 expression in Ctenopharyngodon idellus kidney (CIK) cells.
Krüppel-like factor 2a (KLF2A) suppresses GCRV replication by upregulating serpinc1 expression in Ctenopharyngodon idellus kidney (CIK) cells.
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DOI：
10.1016/j.fsi.2022.11.017
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发表时间：
2022-11
影响因子：
4.7
通讯作者：
Yangyu Li;Liangming Chen;Yangyang Li;Cheng Yang;Bin Gui;Yongming Li;L. Liao;Zuo-Yani Zhu
中科院分区：
文献类型：
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作者：
Yangyu Li;Liangming Chen;Yangyang Li;Cheng Yang;Bin Gui;Yongming Li;L. Liao;Zuo-Yani Zhu
Krüppel 样因子 2a (KLF2A) 是 krüppel 样家族的转录因子，参与调节免疫分子并与病毒感染相关。然而，KLF2A 在鱼类病毒感染期间的功能仍不清楚。本研究以草鱼（Ctenopharyngodon idellus）为对象，预测KLF2A调控的靶基因。结果表明，候选靶基因包括serpin基因家族的4个成员（serpinb1l2、serpinc1、serpinh1a和serpinh1b）。双荧光素酶实验表明klf2a正向调节serpinc1的表达。 C 中剂量依赖性 klf2a 过表达。 idelluskidney (CIK) 细胞显着上调serpinc1 的表达。 CIK细胞中过表达klf2aorserpinc1可激活干扰素反应并抑制草鱼呼肠孤病毒（GCRV）复制。Klf2a和serpinc1共表达抑制GCRV复制。这些结果表明klf2a上调serpinc1mRNA表达，促进1型干扰素反应，并抑制GCRV感染。这项研究提供了对 KLF2A 在鱼类宿主病毒相互作用中的调节作用和生物学功能的见解。
Krüppel-like factor 2a (KLF2A), a transcription factor of the krüppel-like family, is involved in regulating the immune molecules and is associated with viral infection. However, the function of KLF2A during viral infections in fish remains unclear. In this study, grass carp (Ctenopharyngodon idellus) was used to predict the target genes regulated by KLF2A. The results showed that the candidate target genes included four members of the serpin gene family (serpinb1l2,serpinc1,serpinh1a, andserpinh1b). Dual-luciferase experiments showed thatklf2apositively regulatesserpinc1expression. Dose-dependentklf2aoverexpression inC. idelluskidney (CIK) cells significantly upregulated the expression ofserpinc1. Overexpressingklf2aorserpinc1in CIK cells activated interferon responses and suppressed grass carp reovirus (GCRV) replication.Klf2aandserpinc1co-expression inhibited GCRV replication. These results show thatklf2aupregulatesserpinc1mRNA expression, promotes type 1 interferon responses, and suppresses GCRV infection. This study provides insights into the regulatory role and biological functions of KLF2A in host-virus interactions in fish.