Kinetic studies of the enzymatic dopamine beta-hydroxylation reaction.
Kinetic studies of the enzymatic dopamine beta-hydroxylation reaction.
复制标题
酶促多巴胺β-羟基化反应的动力学研究。
DOI：
10.1021/bi00848a005
复制
发表时间：
1968
期刊：
影响因子：
2.9
通讯作者：
T. Garvey
中科院分区：
文献类型：
--
作者：
M. Goldstein;T. H. Joh;T. Garvey
方法多巴胺(3-羟基酶)按上述方法制备(Levin等人，1960年)。该酶如先前在程序A中描述的那样被纯化到第二DEAE柱洗脱阶段(Goldstein等人，1965)。在一些实验中，使用了基本均一的酶制剂。该酶制剂是按照Friedman和Kaufman(1965)的方法制备的。以多巴胺为底物，用荧光法(Von)测定酶活性
MethodsDopamine (3-hydroxylase was prepared as previously described (Levin et al., 1960). The enzyme was purified to the second DEAE column eluate stage as previouslydescribed in procedure A (Goldstein et al., 1965). In some experiments an essentially homogeneous enzyme preparation was used. This enzyme preparation was prepared by the procedure of Friedman and Kaufman (1965). When dopamine was used as a substrate the enzyme activity was assayed fluorimetrically (Von