Pyocyanin isolated from a marine microbial population: Synergistic production between two distinct bacterial species and mode of action
Pyocyanin isolated from a marine microbial population: Synergistic production between two distinct bacterial species and mode of action
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DOI：
10.1016/j.chembiol.2006.10.012
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发表时间：
2006-12-01
影响因子：
--
通讯作者：
Watanabe, Coran M. H.
中科院分区：
文献类型：
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作者：
Angell, Scott;Bench, Bennie J.;Watanabe, Coran M. H.
对从夏威夷群岛收集的海洋微生物种群进行抗菌活性筛选。从混合细胞培养物中鉴定出蓝色代谢物，但在纯培养物中产生并不明显。提出了旨在探测微生物协同作用的实验。提供了蓝色天然产物绿脓素的完整表征，包括对化学文献和酵母转录组分析中误报的分子的 H-1 和 C-13-NMR 归属进行的更正。转录效应与该化合物作为氧化应激和损伤诱导剂的据称作用一致，并说明了该方法揭示天然产物主要生物/细胞效应的整体潜力。这里概述的实验可以作为鉴定微生物群落产生的天然产物并研究它们各自相互作用的通用范例。
Marine microbial populations collected from the Hawaiian Islands were screened for antimicrobial activity. A blue metabolite was identified from mixed cell cultures, but production was not evident in pure cultures. Experiments designed to probe the synergistic role of the microorganisms are presented. Full characterization of the blue natural product, pyocyanin, is provided including corrections made to H-1 and C-13-NMR assignments of the molecule misreported in the chemical literature and yeast transcriptome analysis. The transcriptional effects were consistent with the compound's purported role as an inducer of oxidative stress and damage and illustrates the overall potential of the method to reveal the primary biological/cellular effects of a natural product. The experiments outlined here might serve as a general paradigm for identification of natural products arising from microbial communities and investigation of their respective interactions.