Comparison of DNA damage and repair following radiation challenge in buccal cells and lymphocytes using single-cell gel electrophoresis
Comparison of DNA damage and repair following radiation challenge in buccal cells and lymphocytes using single-cell gel electrophoresis
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DOI：
10.1080/09553000410001723866
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发表时间：
2004-07-01
影响因子：
2.6
通讯作者：
Fenech, M
中科院分区：
文献类型：
--
作者：
Dhillon, VS;Thomas, P;Fenech, M
目的：建立一种可重复的单细胞凝胶电泳检测颊粘膜和舌下脱落细胞DNA损伤和修复的方法。材料和方法：用递增剂量的γ射线照射6个个体（3男3女，年龄34-45岁）的颊粘膜和舌下脱落细胞及淋巴细胞。DNA链断裂和DNA修复测定使用单细胞凝胶电泳assay.Results：基线DNA链断裂显着大于颊粘膜和舌下细胞相比，淋巴细胞。颊粘膜和舌下细胞在2或4戈伊γ射线诱导DNA链断裂方面没有差异。然而，与淋巴细胞相比，它们在γ射线诱导的DNA链断裂中表现出较小的增加（比淋巴细胞低32-53%; ANOVA p
Purpose: To develop a reproducible single-cell gel electrophoresis assay for DNA damage and repair in buccal mucosa and sublingual exfoliated cells.Materials and methods: Buccal mucosa and sublingual cells and lymphocytes from six individuals (three males, three females, aged 34-45 years) were challenged with increasing doses of gamma-rays. DNA strand breaks and DNA repair were measured using the single-cell gel electrophoresis assay.Results: Baseline DNA strand breaks were significantly greater in buccal mucosa and sublingual cells compared with lymphocytes. Buccal mucosa and sublingual cells did not differ from each other with respect to induction of DNA strand breaks by 2 or 4 Gy gamma-rays. However, they showed a smaller increase in gamma-ray-induced DNA strand breaks compared with lymphocytes (32-53% less than lymphocytes; ANOVA p