Fast functionalization of silver decahedral nanoparticles with aptamers for colorimetric detection of human platelet-derived growth factor-BB.
Fast functionalization of silver decahedral nanoparticles with aptamers for colorimetric detection of human platelet-derived growth factor-BB.
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DOI：
10.1016/j.aca.2014.04.034
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发表时间：
2014-06
影响因子：
6.2
通讯作者：
Hui Li;Yue Zhu;S. Dong;Weibing Qiang;Liang Sun;Danke Xu
中科院分区：
文献类型：
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作者：
Hui Li;Yue Zhu;S. Dong;Weibing Qiang;Liang Sun;Danke Xu
建立了基于核酸适体-银十面体纳米粒子（Ag10NPs-aptamer）的蛋白质检测方法。采用光化学方法合成Ag10NPs。Ag10NPs的优点是耐NaCl，这有利于银纳米粒子与各种ssDNA的功能化。与传统方法相比，可以在2小时内将适体附着在Ag10NPs上。以人血小板衍生生长因子bb （PDGF-BB）为模型蛋白，检测Ag10NPs上适配体的结合能力。我们的数据表明，适体- ag10nps偶联物能够成功检测人PDGF-BB。此外，我们开发了一种基于适体ag10nps共轭物的比色传感器来检测PDGF-BB。结果显示血清中PDGF-BB浓度（5 ng mL−1 ~ 200 ng mL−1）与ΔOD呈线性关系，具有良好的检测特异性。因此，基于适配体- ag10nps偶联物的传感器具有高效、灵敏的特点，具有广阔的开发应用前景。
Aptamer-silver decahedral nanoparticles (Ag10NPs-aptamer) based detection was developed for protein. Ag10NPs were synthesized by photochemical method. The advantage of Ag10NPs was its tolerance of NaCl which facilitates the functionalization of silver nanoparticles with all kinds of ssDNA. Attaching aptamers to Ag10NPs could be achieved within 2 h, much faster than traditional methods. Human platelet-derived growth factor-BB (PDGF-BB) was used as a model protein to test the binding capacity of aptamers attached on Ag10NPs. Our data showed that the aptamer-Ag10NPs conjugates were successful in detecting human PDGF-BB. Furthermore, we developed an aptamer-Ag10NPs conjugates-based colorimetric sensor to detect PDGF-BB. The results showed a linear relationship between PDGF-BB concentrations (5 ng mL−1–200 ng mL−1) and ΔOD with excellent detection specificity in serum. Therefore, the sensor based on aptamer-Ag10NPs conjugates was highly effective and sensitive and had great promise for further development and applications.