Ribo-attenuators: novel elements for reliable and modular riboswitch engineering.
Ribo-attenuators: novel elements for reliable and modular riboswitch engineering.
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DOI：
10.1038/s41598-017-04093-x
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发表时间：
2017-07-04
影响因子：
4.6
通讯作者：
Rothschild LJ
中科院分区：
文献类型：
--
作者：
Folliard T;Mertins B;Steel H;Prescott TP;Newport T;Jones CW;Wadhams G;Bayer T;Armitage JP;Papachristodoulou A;Rothschild LJ
核糖开关是一种结构基因调控元件，它直接将小分子的感知与基因表达联系起来。由于它们能够感知广泛的小分子并调节基因表达，因此在合成生物学和生物制造领域具有相当大的应用潜力。尽管进行了十多年的研究，但它们还没有达到这种巨大的潜力，因为它们还不能被视为模块化组件。这是由于几个限制，包括对遗传背景变化的敏感性、低可调整性和性能的可变性。为了克服与核糖开关相关的困难，我们设计并引入了一种新的遗传元件，称为细菌中的核糖体衰减器。这种遗传因素允许可预测的调整，不受上下文变化的影响，并减少表达差异。核糖衰减器允许将核糖开关视为真正的模块化和可调组件，从而提高了它们在广泛应用中的可靠性。
Riboswitches are structural genetic regulatory elements that directly couple the sensing of small molecules to gene expression. They have considerable potential for applications throughout synthetic biology and bio-manufacturing as they are able to sense a wide range of small molecules and regulate gene expression in response. Despite over a decade of research they have yet to reach this considerable potential as they cannot yet be treated as modular components. This is due to several limitations including sensitivity to changes in genetic context, low tunability, and variability in performance. To overcome the associated difficulties with riboswitches, we have designed and introduced a novel genetic element called a ribo-attenuator in Bacteria. This genetic element allows for predictable tuning, insulation from contextual changes, and a reduction in expression variation. Ribo-attenuators allow riboswitches to be treated as truly modular and tunable components, thus increasing their reliability for a wide range of applications.