Contributions of the effector gene hopQ1-1 to differences in host range between Pseudomonas syringae pv. phaseolicola and P-syringae pv. tabaci
Contributions of the effector gene hopQ1-1 to differences in host range between Pseudomonas syringae pv. phaseolicola and P-syringae pv. tabaci
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DOI：
10.1111/j.1364-3703.2009.00577.x
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发表时间：
2009-11-01
影响因子：
4.9
通讯作者：
Vinatzer, Boris A.
中科院分区：
文献类型：
--
作者：
Ferrante, Patrizia;Clarke, Christopher R.;Vinatzer, Boris A.
研究烟草（Nicotiana sp.）病原菌假单胞菌烟粉虱，选择了7个菌株的特点，首先是多位点序列分型（MLST），以确定其系统发育的亲和力。MLST显示，所有菌株代表一个紧密的系统发育组，并且具有完全测序的基因组的最密切相关的菌株是菜豆（菜豆）病原体P. phaseolicola 1448A.利用针对21株烟草黑胫病菌的引物，phaseolicola 1448 A效应基因，确定P. phaseolicola 1448 A与所有供试的P. lingae pv.烟粉虱品系11个效应子中的6个未能从P.烟粉虱菌株分别在一个P.烟粉虱株。虽然5种效应子对转基因水稻的表型、植株生长和病害严重程度均无影响。烟粉虱在菜豆中表达hopQ 1 -1（Pph 1448 A）的量显著增加，而在烟草中表达量降低。我们的结论是，hopQ 1 -1已保留在P. phaseolicola 1448 A，因为该效应子抑制菜豆中的免疫，而hopQ 1 -1在P.烟粉虱菌株，因为它触发烟草属的防御。这提供了证据表明，微调效应库在宿主适应过程中导致在非宿主物种的毒力的同时减少。
P>To study the role of type III-secreted effectors in the host adaptation of the tobacco (Nicotiana sp.) pathogen Pseudomonas syringae pv. tabaci, a selection of seven strains was first characterized by multilocus sequence typing (MLST) to determine their phylogenetic affinity. MLST revealed that all strains represented a tight phylogenetic group and that the most closely related strain with a completely sequenced genome was the bean (Phaseolus vulgaris) pathogen P. syringae pv. phaseolicola 1448A. Using primers designed to 21 P. syringae pv. phaseolicola 1448A effector genes, it was determined that P. syringae pv. phaseolicola 1448A shared at least 10 effectors with all tested P. syringae pv. tabaci strains. Six of the 11 effectors that failed to amplify from P. syringae pv. tabaci strains were individually expressed in one P. syringae pv. tabaci strain. Although five effectors had no effect on phenotype, growth in planta and disease severity of the transgenic P. syringae pv. tabaci expressing hopQ1-1(Pph1448A) were significantly increased in bean, but reduced in tobacco. We conclude that hopQ1-1 has been retained in P. syringae pv. phaseolicola 1448A, as this effector suppresses immunity in bean, whereas hopQ1-1 is missing from P. syringae pv. tabaci strains because it triggers defences in Nicotiana spp. This provides evidence that fine-tuning effector repertoires during host adaptation lead to a concomitant reduction in virulence in non-host species.