Increased bladder activity is associated with elevated oxidative stress markers and proinflammatory cytokines in a rat model of atherosclerosis-induced chronic bladder ischemia
Increased bladder activity is associated with elevated oxidative stress markers and proinflammatory cytokines in a rat model of atherosclerosis-induced chronic bladder ischemia
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DOI：
10.1002/nau.21191
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发表时间：
2012-01-01
影响因子：
2
通讯作者：
Yamaguchi, Osamu
中科院分区：
文献类型：
--
作者：
Nomiya, Masanori;Sagawa, Koji;Yamaguchi, Osamu
目的在大鼠模型中进一步表征动脉粥样硬化诱导的慢性膀胱缺血对膀胱功能的影响以及氧化应激标志物和促炎细胞因子的相关变化。方法：成年SD雄性大鼠随机分为三组（动脉内皮损伤组：AI组、假手术组、未处理组）。AI组（n = 14）进行髂动脉内皮损伤，并接受2%的胆固醇饮食。假手术组（n 12）行假手术，并给予2%胆固醇饮食。初治组（n 12）给予常规饮食。8周后，在无麻醉或约束的情况下进行膀胱测压（CMG）。在每组的膀胱中，定量氧化应激标志物（8-羟基-20-脱氧鸟苷：8-OHdG;丙二醛：MDA）和促炎细胞因子（IL-8样细胞因子CXCL 1/CINC-1、TNF-α、IL-6）。还对髂动脉进行了组织学检查。结果：8周时，三组动物的体重和膀胱湿重无显著性差异。AI组排尿间隔较其他两组明显缩短，但排尿时最大压力无明显变化。AI组髂动脉显示球囊损伤部位内膜增厚和弥漫性中膜纤维化。AI组的氧化应激标志物和促炎细胞因子水平显著高于其他组。结论：氧化应激和炎症反应可能是膀胱过度活动症发生发展的关键因素
Aims To further characterize, in a rat model, the effects of atherosclerosis-induced chronic bladder ischemia on bladder function and associated changes in oxidative stress markers and proinflammatory cytokines. Methods: Adult Sprague-Dawley male rats were divided into three groups (arterial endothelial injury: AI, sham, nai " ve). The AI group (n = 14) underwent endothelial injury of the iliac arteries and received a 2% cholesterol diet. The sham group (n 12) underwent sham operation and received a 2% cholesterol diet. The nai " ve group (n 12) received a regular diet. After 8 weeks, cystometrograms (CMG) without anesthesia or restraint were performed. In bladders from each group, oxidative stress markers (8-hydroxy-20-deoxyguanosine: 8-OHdG; malondialdehyde: MDA) and proinflammatory cytokines (IL-8 like cytokine CXCL1/CINC-1, TNF-alpha, IL-6) were quantified. Histological examination of the iliac arteries was also performed. Results: At 8 weeks, the body and bladder wet weights were not significant different among the three groups. The micturition interval in the AI group decreased significantly compared with those in the other two groups, but maximum pressure during micturition did not change. The iliac arteries in the AI group revealed thickening of intima as well as diffuse media fibrosis at the sites of balloon injury. The levels of oxidative stress markers and proinflammatory cytokines were significantly higher in the AI than in the other groups. Conclusion: Oxidative stress and inflammation may be key factors in the development of bladder overactivity