Neurogenic astrocytes transplanted into the adult mouse lateral ventricle contribute to olfactory neurogenesis, and reveal a novel intrinsic subependymal neuron.
Neurogenic astrocytes transplanted into the adult mouse lateral ventricle contribute to olfactory neurogenesis, and reveal a novel intrinsic subependymal neuron.
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移植到成年小鼠侧脑室的神经源性星形胶质细胞有助于嗅觉神经发生，并揭示了一种新的内在室管膜下神经元。
DOI：
10.1016/j.neuroscience.2006.05.051
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发表时间：
2006
期刊：
影响因子：
3.3
通讯作者：
Steindler,DA
中科院分区：
文献类型：
--
作者：
Zheng,T;Marshall2nd,GP;Laywell,ED;Steindler,DA
空间和时间限制的神经源性星形胶质细胞群体可以在体外产生多能神经球。为了研究神经源性星形胶质细胞对体内分化线索的反应能力，我们将培养的新生小脑星形胶质细胞直接移植到成年小鼠的侧脑室中，使其进入室管膜下区（SEZ）。在这里，我们报告三个事件，遵循这样的移植。1)供体细胞附着于心室周围结构，并形成穿透心室壁的“肿瘤样”球体。这些附着的球体可以持续数月，因为它们产生了渗透到前脑实质的细胞“克隆”。2)许多供体细胞进入吻侧迁移流并迁移到嗅球，其中一小部分分化为嗅觉中间神经元。3)最后，在SEZ内，一些供体细胞形成了似乎与SEZ神经元前体链相互作用的细胞簇，并且一些供体细胞分化成独特的神经元，其具有精确地限制在室管膜层和纹状体之间的广泛的珠状突起。正常的SEZ解剖的进一步分析显示，土著神经元具有相同的形态，其中一些是由5-HT+纤维接触，我们建议代表一个迄今为止的未知功能的，内在的SEZ神经元。这些结果表明，培养的星形胶质细胞来源于非SEZ脑区可以以不同的方式响应于成人侧脑室和SEZ提供的体内线索，分化成神经元，最终栖息在嗅球和SEZ适当。
Spatially and temporally restricted populations of neurogenic astrocytes can generate multipotent neurospheres in vitro. To examine the ability of neurogenic astrocytes to respond to in vivo differentiation cues within a germinal matrix, we provided cultured neonatal cerebellar astrocytes access to the subependymal zone (SEZ) by grafting them directly into the lateral ventricle of adult mice. Here we report three events that follow such transplants. 1) Donor cells attach to periventricular structures, and form “neoplastic-like” spheres that penetrate the ventricular wall. These attached spheres can persist for months, as they give rise to “clones” of cells that infiltrate forebrain parenchyma. 2) Many donor cells enter the rostral migratory stream and migrate into the olfactory bulb where a small percentage differentiates as olfactory interneurons. 3) Finally, within the SEZ, some donor cells formed cell clusters that appear to interact with the SEZ neuronal precursor chains, and some donor cells differentiate into distinctive neurons with extensive, beady projections precisely confined between the ependymal layer and the striatum. Further analysis of normal SEZ anatomy reveals indigenous neurons with identical morphologies—some of which are contacted by 5-HT+ fibers—that we propose represent a heretofore uncharacterized, intrinsic SEZ neuron of unknown function. These results suggest that cultured astrocytes derived from non-SEZ brain regions can respond in different ways to in vivo cues provided by the adult lateral ventricle and SEZ by differentiating into neurons that eventually inhabit both the olfactory bulb and SEZ proper.