Ethanol exposure alters zebrafish development: A novel model of fetal alcohol syndrome
Ethanol exposure alters zebrafish development: A novel model of fetal alcohol syndrome
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DOI：
10.1016/j.ntt.2004.06.011
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发表时间：
2004-11-01
影响因子：
2.9
通讯作者：
Saszik, S
中科院分区：
文献类型：
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作者：
Bilotta, J;Barnett, JA;Saszik, S
产前暴露于酒精已被证明会产生明显的身体和行为症状，称为胎儿酒精综合征（FAS）。此外，据信，低浓度和/或短时间的酒精暴露可以产生更微妙的影响。本研究的目的是研究胚胎乙醇暴露对斑马鱼（Danio rerio）的影响，以确定该物种是否是研究FAS的可行动物模型。在不同浓度的乙醇（1.5%和2.9%）和暴露时间（例如，受精后0-8、6-24、12-24和48-72 h; HPF）;解剖学测量，包括眼球直径和心率，在各组之间进行比较。结果发现，在最高浓度的乙醇（2.9%），有更多的异常身体扭曲和显着高于其他组的死亡率。胚胎暴露于浓度为1.5%的乙醇较短的持续时间（0-8 hpf）表现出更微妙的影响，如显着更小的眼睛直径和较低的心率比对照组。这些结果表明，胚胎酒精暴露会影响外部和内部的身体发育，这些影响的严重程度是一个功能的乙醇量和乙醇暴露的时间。因此，斑马鱼代表了一个有用的模型，用于检查胚胎暴露于乙醇对发育的影响的基本问题。(C)2004年爱思唯尔公司All rights reserved.
Prenatal exposure to alcohol has been shown to produce the overt physical and behavioral symptoms known as fetal alcohol syndrome (FAS) in humans. Also, it is believed that low concentrations and/or short durations of alcohol exposure can produce more subtle effects. The purpose of this study was to investigate the effects of embryonic ethanol exposure on the zebrafish (Danio rerio) in order to determine whether this species is a viable animal model for studying FAS. Fertilized embryos were reared in varying concentrations of ethanol (1.5% and 2.9%) and exposure times (e.g., 0-8, 6-24, 12-24, and 48-72 h postfertilization; hpf); anatomical measures including eye diameter and heart rate were compared across groups. Results found that at the highest concentration of ethanol (2.9%), there were more abnormal physical distortions and significantly higher mortality rates than any other group. Embryos exposed to ethanol for a shorter duration period (0-8 hpf) at a concentration of 1.5% exhibited more subtle effects such as significantly smaller eye diameter and lower heart rate than controls. These results indicate that embryonic alcohol exposure affects external and internal physical development and that the severity of these effects is a function of both the amount of ethanol and the timing of ethanol exposure. Thus, the zebrafish represents a useful model for examining basic questions about the effects of embryonic exposure to ethanol on development. (C) 2004 Elsevier Inc. All rights reserved.