AUG-proximal nucleotides regulate protein synthesis in Leishmania tropica.
AUG-proximal nucleotides regulate protein synthesis in Leishmania tropica.
复制标题
AUG-近端核苷酸调节热带利什曼原虫的蛋白质合成。
DOI：
10.1111/j.1365-2958.2006.05228.x
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Mensa-Wilmot,Kojo
中科院分区：
文献类型：
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作者：
Stanton,JulieD;Mensa-Wilmot,Kojo
Gene expression in theLeishmaniais controlled post‐transcriptionally, and is likely to be impacted by both 5′ and 3′ untranslated regions (UTRs). We have investigated the effects of trinucleotides in the AUG‐proximal region (APR) (i.e. positions −3 to −1 upstream of an AUG) on two reporter genes in the context of an endogenous intergenic region ofLeishmania tropica.The effects of APRs on protein expression were determined in stable transfectantsin vivo. Three APRs, namely, C−3C−2C−1, ACC and GCC, yielded robust translation, whereas GTA produced low amounts of proteins. A purine at −3 of an APR was not crucial for efficient translation. Steady‐state level of reporter mRNA did not correlate directly with the amount of protein detected. Polysome analysis revealed that APRs modulate translation, at least in part, by influencing mRNA association with ribosomes. An analysis of genomic UTRs inL. majorshowed that (i) the consensus APR is N−3N−2C−1(where N = any nucleotide), and (ii) the most frequently used APRs include ACA, ACC, ATC, GCC, GCG, GTC and CAC, some of which were translation enhancers in our experimental studies.
