FERONIA Is a Key Modulator of Brassinosteroid and Ethylene Responsiveness in Arabidopsis Hypocotyls
FERONIA Is a Key Modulator of Brassinosteroid and Ethylene Responsiveness in Arabidopsis Hypocotyls
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DOI：
10.1093/mp/ssq015
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发表时间：
2010-05-01
期刊：
影响因子：
27.5
通讯作者：
Larsen, Paul B.
中科院分区：
文献类型：
--
作者：
Deslauriers, Stephen D.;Larsen, Paul B.
乙烯是一种复杂的信号转导途径，人们对其进行了深入的分析，部分原因是它对包括果实成熟和组织衰老在内的园艺现象的表现具有重要意义。为了进一步了解这一途径是如何调控的，我们对乙烯反应增强的拟南芥突变体进行了筛选。由此，一个突变体被鉴定为具有黑暗生长的下胚轴，在乙烯感受抑制剂AgNO3存在下与Col-0 wt难以区分，但即使在低水平的乙烯下也具有极高的响应性。基于地图的突变克隆显示，T-DNA插入在受体样激酶Feronia的编码序列中，这是正常的花粉管接收和细胞伸长所必需的，目前尚不清楚。与之前的报告不同，对我们的费洛尼亚基因敲除突变体的分析表明，它也改变了对油菜素类固醇的反应，黄化的FER-2幼苗在促进下胚轴伸长方面部分对油菜素类固醇不敏感。我们的结果表明，依赖费洛尼亚的油菜素类固醇反应有助于拮抗乙烯对黄化幼苗下胚轴生长的影响，适当的油菜素类固醇信号的丢失破坏了这种平衡，导致乙烯对下胚轴缩短的影响更大。
Ethylene signaling is a complex pathway that has been intensively analyzed partly due to its importance to the manifestation of horticultural phenomena, including fruit ripening and tissue senescence. In order to further our understanding of how this pathway is regulated, a screen for Arabidopsis mutants with increased ethylene response was conducted. From this, a mutant was identified as having a dark-grown hypocotyl that is indistinguishable from Col-0 wt in the presence of the ethylene perception inhibitor AgNO3, yet has extreme responsiveness to even low levels of ethylene. Map-based cloning of the mutation revealed a T-DNA insertion in the coding sequence of the receptor-like kinase FERONIA, which is required for normal pollen tube reception and cell elongation in a currently unknown capacity. In contrast to a previous report, analysis of our feronia knockout mutant shows it also has altered responsiveness to brassinosteroids, with etiolated fer-2 seedlings being partially brassinosteroid insensitive with regard to promotion of hypocotyl elongation. Our results indicate that FERONIA-dependent brassinosteroid response serves to antagonize the effect of ethylene on hypocotyl growth of etiolated seedlings, with loss of proper brassinosteroid signaling disrupting this balance and leading to a greater impact of ethylene on hypocotyl shortening.