Drop Method as a Quantitative Bioassay Method of Chemotaxis of Aphanomyces cochlioides Zoospore
Drop Method as a Quantitative Bioassay Method of Chemotaxis of Aphanomyces cochlioides Zoospore
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滴法作为线状丝丝菌游动孢子趋化性的定量生物测定方法
DOI：
10.3186/jjphytopath.64.175
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
S. Tahara
中科院分区：
文献类型：
--
作者：
T. Takayama;J. Mizutani;S. Tahara
滴法使用氟化烃（FC-72）成功地应用于作为定量分析，以确定的阈值浓度的旋孢菌素A，一个强大的丝囊孢游动孢子引诱剂最初从菠菜根分离。该方法揭示了被吸引到含有旋孢亲和素A的液滴的游动孢子的数量随时间增加，直到将液滴注射到游动孢子悬浮液中后约60至90秒。在此浓度以上，旋藻亲脂素A能够作为游动孢子引诱剂起作用，其阈值浓度被确定为约100 mg/L。3.0×10-9M。有趣的是，游动孢子被含有浓度大于1.1×10- 7 M的螺旋藻素A的液滴吸引，聚集在液滴表面，这一行为与在寄主植物根表面观察到的行为非常相似。这一结果表明，点滴法不仅可作为一种定量测定趋化性的方法，而且可作为一种研究游动孢子聚集机制的技术。
A drop method using fluorinated hydrocarbon (FC-72) was successfully applied as a quantitative assay to determine the threshold concentration of cochliophilin A, a potent Aphanomyces cochlioides zoospore attractant originally isolated from spinach roots. This method revealed that the number of zoospores attracted to droplets containing cochliophilin A increased with time, until approximately 60 to 90sec after injection of the droplet into a zoospore suspension. The threshold concentration, above which cochliophilin A was able to function as a zoospore attractant, was determined to be ca. 3.0×10-9M. Interestingly, zoospores attracted to droplets containing cochliophilin A at a concentration greater than 1.1×10-7M massed on the surface of the droplets, a behavior that was quite similar to that observed on the root surface of host plants. This result suggested that the drop method would be useful not only as a quantitative assay of chemotaxis, but also as a technique to investigate the mechanism of zoospore massing.