Elution of glycoproteins from replicas of sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels.
Elution of glycoproteins from replicas of sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels.
复制标题
从十二烷基硫酸钠-聚丙烯酰胺凝胶电泳凝胶的复制品中洗脱糖蛋白。
DOI：
10.1002/elps.1150190214
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Summers,DF
中科院分区：
文献类型：
--
作者：
Szewczyk,B;Pilat,Z;Bienkowska-Szewczyk,K;Summers,DF
描述了一种从聚偏二氟乙烯（PVDF）膜上的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）复制品中洗脱糖蛋白的方法。通过SDS-PAGE分离十种模型糖蛋白，然后电转移到PVDF膜上。可逆染色后，用SDS/Triton X-100混合物（pH 9）或盐酸胍/溶血磷脂酰胆碱混合物（pH中性）洗脱糖蛋白条带。对于这两种类型的洗脱液，最终回收率范围为30%以上至约80%。良好的回收率和温和的洗脱条件使该方法适用于聚糖链的结构解析。
A method for the elution of glycoproteins from sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) replicas of gels on polyvinylidene difluoride (PVDF) membranes is described. Ten model glycoproteins were resolved by SDS‐PAGE and then electrotransferred onto PVDF membranes. After reversible staining, glycoprotein bands were eluted with a mixture of SDS/Triton X‐100 at pH 9 or with a mixture of guanidinium hydrochloride/lysophosphatidylcholine at neutral pH. For both types of eluents, the final recoveries ranged from over 30% to about 80%. Good recoveries and mild conditions of elution render the method applicable for the structural elucidation of glycan chains.