Transcutaneous immunisation assisted by low-frequency ultrasound
Transcutaneous immunisation assisted by low-frequency ultrasound
复制标题
DOI：
10.1016/j.ijpharm.2008.10.014
复制
发表时间：
2009-02-23
影响因子：
5.8
通讯作者：
Murdan, Sudaxshina
中科院分区：
文献类型：
--
作者：
Dahlan, Afendi;Alpar, H. Oya;Murdan, Sudaxshina
已知低频超声应用可增加皮肤对大分子（如疫苗）的渗透性，并使经皮免疫成为可能。十二烷基硫酸钠（SDS）-一种皮肤刺激物，通常以1%（w/v）的浓度包含在耦合介质中。因为已经发现这能增强皮肤渗透性。在本文中，我们首次展示了在没有SDS的情况下低频超声辅助经皮免疫的可行性。抗体滴度受实验条件的强烈影响。偶联介质中SDS的存在增加了抗体滴度，尽管0.5%（w/v）的较低浓度产生的滴度比常用的1%（w/v）高得多，但引起的皮肤损伤较少。与20%的占空比相比，10%的较低超声占空比产生更高的抗体滴度，尽管引起更低的皮肤损伤。皮肤损伤和免疫应答之间缺乏相关性表明，皮肤对局部应用抗原的渗透性增强（如皮肤完整性的变化所示）不是低频超声辅助皮肤免疫的主要机制。(C)2008 Elsevier B. V.保留所有权利。
Low-frequency ultrasound application is known to increase the skin's permeability to large molecules such as vaccines, and to enable transcutaneous immunisation. Sodium dodecyl sulphate (SDS) - a skin irritant is often included in the coupling medium at 1% (w/v). as this has been found to enhance skin permeability. In this paper we show, for the first time, the feasibility of low-frequency ultrasound-assisted transcutaneous immunisation in the absence of SDS. Antibody titres were strongly influenced by experimental conditions. SDS presence in the coupling medium increased antibody titres, though a lower concentration of 0.5% (w/v) generated much higher titres than the commonly used 1% (w/v), despite causing less skin damage. A lower ultrasound duty cycle of 10% generated higher antibody titres than a duty cycle of 20%, also despite causing lower skin damage. Such lack of correlation between skin damage and immune responses indicates that enhancement of skin permeability to topically applied antigen (as indicated by changes in skin integrity) was not the main mechanism of low-frequency ultrasound-assisted skin immunisation. (C) 2008 Elsevier B.V. All rights reserved.