Canonical Wnt signaling is required for Panax notoginseng saponin-mediated attenuation of the RANKL/OPG ratio in bone marrow stromal cells during osteogenic differentiation
Canonical Wnt signaling is required for Panax notoginseng saponin-mediated attenuation of the RANKL/OPG ratio in bone marrow stromal cells during osteogenic differentiation
复制标题
DOI：
10.1016/j.phymed.2012.06.002
复制
发表时间：
2012-08-15
期刊：
影响因子：
7.9
通讯作者：
Du, Shi-xin
中科院分区：
文献类型：
--
作者：
Chen, Bin;Li, Xue-dong;Du, Shi-xin
三七总皂苷（PNS）对骨髓基质细胞（BMSCs）的成骨分化具有调节作用。在本研究中，我们研究了三七总皂苷是否可以通过调节Wnt/β-catenin信号通路促进BMSCs的成骨分化，这与BMSCs的成骨有关。我们发现三七总皂苷可增强OPG、β-catenin和cyclin D1的mRNA表达，同时降低RANKL和PPAR γ 2的mRNA表达。PNS对BMSCs的作用可被经典的Wnt/β-catenin信号抑制剂DKK-1逆转（或部分逆转）。提示三七总皂苷通过促进BMSCs增殖和成骨分化促进骨形成，并通过Wnt/beta-catenin信号通路降低RANKL/OPG表达，从而降低骨吸收，保护骨骼系统。(C)2012 Elsevier GmbH. All rights reserved.
Panax notoginseng saponins (PNS) are known to regulate the osteogenic differentiation of bone marrow stromal cells (BMSCs). In the present study, we investigated whether PNS could promote the osteogenic differentiation of BMSCs through modulating the Wnt/beta-catenin signaling pathways, which are implicated in BMSCs osteogenesis. We found that PNS enhanced the mRNA expression of OPG, beta-catenin, and cyclin D1 while decreased the mRNA expression of RANKL and PPAR gamma 2. The actions of PNS on BMSCs were reversed (or partially) by DKK-1, a classical inhibitor of Wnt/beta-catenin signaling. These results suggest that PNS stimulating bone formation by promoting the proliferation and osteogenic differentiation of BMSCs, and could also protect the skeletal system by decreasing bone resorption through reduction of RANKL/OPG expression via Wnt/beta-catenin signaling pathways. (C) 2012 Elsevier GmbH. All rights reserved.