Phosphorylation of the JAK2-STAT5 pathway in response to acute aerobic exercise
Phosphorylation of the JAK2-STAT5 pathway in response to acute aerobic exercise
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DOI：
10.1249/mss.0b013e3181690760
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发表时间：
2008-06-01
期刊：
影响因子：
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通讯作者：
Morrison, Ron F.
中科院分区：
文献类型：
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作者：
Consitt, Leslie A.;Wideman, Laurie;Morrison, Ron F.
目的：研究急性有氧运动对人骨骼肌中JAK2-STAT5信号通路的影响。方法：11名年轻男性(22.5+/-0.6，均值+/-SE)，健康，接受有氧运动训练，在70%最大摄氧量下骑行30min。每隔10至15分钟采集一次血样，分析人类生长激素、免疫功能性生长激素(IF)生长激素结合蛋白和胰岛素样生长因子I(IGF-I)。分别于运动前、运动后即刻、运动后30分钟和60分钟取股外侧肌组织标本。分析肌肉样本JAK2和STAT5酪氨酸磷酸化的变化，以及JAK2和STAT5蛋白含量的变化。结果：多变量方差分析显示，与运动前相比，运动后即刻GH和IF GH显著升高(P<；0.001)。运动后即刻、运动后即刻JAK2的磷酸化水平显著升高(P=0.004)。与运动前相比，STAT5磷酸化水平在运动后即刻有升高趋势(P=0.08)，运动后30min显著升高(P=0.002)。肌肉中JAK2和STAT5蛋白含量没有变化。结论：JAK2-STAT5通路在急性有氧运动后被激活，提示运动诱导的生长激素释放可能参与了该通路的激活。
Purpose: The purpose of this study was to determine the phosphorylation of the JAK2-STAT5 pathway in human skeletal muscle in response to acute aerobic exercise. Methods: Eleven young (22.5 +/- 0.6, mean +/- SE), healthy, aerobically trained males performed 30 min of cycling at 70% VO2max. Blood samples were collected at 10- to 15-min intervals and analyzed for human GH, immunofunctional (IF) GH GH binding protein, and insulin-like growth factor I (IGF-I). Muscle biopsies were taken from the vastus lateralis before exercise, immediately after exercise, as well as, 30 and 60 min postexercise. Muscle samples were analyzed for changes in JAK2 and STAT5 tyrosine phosphorylation, as well as changes in JAK2 and STAT5 protein content. Results: Multivariate ANOVA with post hoc comparisons demonstrated that GH and IF GH were significantly elevated immediately after exercise compared with preexercise (P < 0.001). Exercise significantly increased the phosphorylation of JAK2 immediately, after exercise (P = 0.004). A trend toward increasing levels of STAT5 phosphorylation was observed immediately after exercise (P = 0.08) and was significantly elevated 30 min after exercise (P = 0.002), compared with preexercise levels. Muscle JAK2 and STAT5 protein content did not change. Conclusion: The results demonstrate that the JAK2-STAT5 pathway is activated in response to acute aerobic exercise in human skeletal muscle and suggests that the exercise-induced release of GH may play a role in the activation of this pathway.